Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 61, Issue 49, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202210652
Keywords
RNA modification; 5-formylcytidine; chemical labelling; photochemical cyclization; sequencing
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Funding
- National Key R&D Program of China [2020YFA0707901]
- National Natural Science Foundation of China [22022704, 21977097, 32121001, 22271291]
- Chinese Academy of Sciences
- Postdoctoral Innovative Talents Support Program [BX20200337]
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This study developed a sensitive antibody-free method for detecting and sequencing f(5)C in tRNA. The method uses chemical labeling and fluorescence signal, allowing high-resolution detection of f(5)C with high selectivity and sensitivity.
5-Formylcytidine (f(5)C) is one of the epigenetic nucleotides in tRNA. Despite the evident importance of f(5)C in gene expression regulation, little is known about its exact amount and position. To capture this information, we developed a modification-specific functionalization with a semi-stabilized ylide. The chemical labelling exhibited a high selectivity towards f(5)C and allowed distinction from similar 5-formyluridine. We realized a detection strategy based on the fluorescence signal of the cyclization product 4,5-pyridin-2-amine-cytidine paC, which exhibited a high quantum yield. The results clearly identified f(5)C with a limit of detection at 0.58 nM. This method altered the hydrogen bonding activities of f(5)C and modulated its reverse transcription signature in its sequencing profile. We showed that f(5)C can be detected from tRNA segments with a single-base resolution. Taken together, this approach is a sensitive, antibody-free, and applicable detection and sequencing method for f(5)C-containing RNA.
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