Journal
ANALYTICAL CHEMISTRY
Volume 94, Issue 36, Pages 12500-12506Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.2c02726
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Funding
- National Natural Science Foundation of China [21874020, 82171474, 81772287, 81371902]
- National Science Foundation for Distinguished Young Scholars of Fujian Province [2020J06019]
- Program for Fujian Youth Talent Support Project [2019B016]
- Program for Fujian Top-notch Innovative Personnel (Fujian Commission Talent) [5]
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In this study, a novel co-reactant-mediated high-performance low-potential Au nano-cluster (AuNC)-based ECL system was successfully developed for the detection of SARS-CoV-2 N protein. The ECL system exhibited higher energy efficiency and quantum yield, with a wide linear range and low detection limit. It showed good reproducibility and stability, making it suitable for the detection of actual serum samples.
Screening high-performance anodic electrochemilu-minescence (ECL) systems with low triggering potential is a promising way to broaden their applications. In addition to electrochemiluminophore, co-reactant also plays an important role in the ECL process, since the oxidation of co-reactants is one of the most important steps in the anodic ECL process. Herein, a novel co-reactant-mediated high-performance low-potential Au nano-cluster (AuNC)-based ECL system has been successfully developed. Benefiting from the isopropyl substitution and hydroxyl addition to the triethylamine (TEA), the BSA-AuNC/2-(diisopropylamino)ethanol (DIPEA-OH) ECL system achieved higher energy efficiency at a lower potential of 0.75 V. In addition, compared with the BSA-AuNC/TEA system, the ECL intensity and quantum yield (phi ECL) with DIPEA-OH as a co-reactant increased 22.34-fold and 13-fold (as high as 68.17%), respectively. Based on the low potential, high phi ECL of the AuNC/DIPEA-OH ECL system, a sandwich-type immunosensor has been constructed for a highly selective SARS-CoV-2 N protein assay. In the absence of any complex signal amplification strategies, the ECL immunosensor for the SARS-CoV-2 N protein detection showed a linear range of 0.001-100 ng/mL and a detection limit of 0.35 pg/mL. Moreover, the ECL platform had good reproducibility and stability and exhibited acceptable detection performance in the detection of actual serum samples. This work established a framework for in-depth design and study of anode ECL co-reactants for AuNCs and other luminophores, and expanded the potential application of ECL sensors in the clinical diagnosis of COVID-19.
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