Rational Design, Synthesis of Fluorescence Probes for Quantitative Detection of Amyloid-β in Alzheimer's Disease Based on Rhodamine-Metal Complex

The efficient detection and monitoring of amyloid-beta plaques (A beta 42) can greatly promote the diagnosis and therapy of Alzheimer's disease (AD). Fluorescence imaging is a promising method for this, but the accurate determination of A beta 42 still remains a challenge. The development of a reliable fluorescent probe to detect A beta 42 is essential. Herein, we report a rational design strategy for A beta 42 fluorescence probes based on rhodamine-copper complexes, Rho1-Cu-Rho4-Cu, among them Rho4-Cu exhibits the best performance including high sensitivity (detection limit = 24 nM), high affinity (K-d = 23.4 nM), and high selectivity; hence, Rho4-Cu is selected for imaging A beta 42 in AD mice, and the results showed that this probe can differentiate normal mice and AD mice effectively.

Automated summary

In this study, a rational design strategy for A beta 42 fluorescence probes based on rhodamine-copper complexes was reported. The results showed that the Rho4-Cu probe exhibited high sensitivity, high affinity, and high selectivity, and was able to effectively differentiate normal mice and AD mice.