Journal
ANALYTICAL CHEMISTRY
Volume 94, Issue 43, Pages 15100-15107Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.2c03285
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Funding
- National Leading Research Lab Program of the National Research Foundation of Korea (NRF) - Korean government (MSIP) [NRF-2022R1A2B5B03001607, NRF-2019R1A5A2026045, NRF-2021R1A6A1A10044950]
- Ajou University Research Fund
- Korea Basic Science Institute (National Research Facilities and Equipment Center) - Ministry of Education [NRF-2021R1A6C103B407, 2019R1A6C1010003]
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In this study, we developed a fluorescent probe that selectively monitors the plasma membrane in live samples, showing bright fluorescence and high selectivity in cells and tissues.
The plasma membrane, which is a phosphoglyceride bilayer at the outer edge of the cell, plays diverse and important roles in biological systems. Visualization of the plasma membrane in live samples is important for various applications in biological functions. We developed an amphiphilic two-photon (TP) fluorescent probe (THQ-Mem) to selectively monitor the plasma membrane in live samples. This probe exhibited red emission (620-700 nm), large TP absorption cross sections (delta max > 790 GM), and high selectivity to the plasma membrane. In cultured cells and in vivo hepatic tissue imaging, THQ-Mem showed bright TPexcited fluorescence (TPEF) and remarkable selectivity for the plasma membrane. Furthermore, simultaneous in vivo imaging with THQ-Mem and a TP lipid droplet probe could serve as an efficient tool to monitor morphological and physiological changes in the plasma membrane and lipid droplets.
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