4.8 Article

Standard-Free Absolute Quantitation of Antibody Deamidation Degradation and Host Cell Proteins by Coulometric Mass Spectrometry

Related references

Note: Only part of the references are listed.
Article Biochemical Research Methods

Recent progress in the analysis of protein deamidation using mass spectrometry

Yujia Ying et al.

Summary: Deamidation is a nonenzymatic and spontaneous posttranslational modification that is strongly associated with aging proteome instability and degenerative diseases. It is also a major cause of degradation in biopharmaceutical proteins. Mass spectrometry analysis is an important tool for accurate characterization of protein deamidation and its related modifications, providing insights into disease pathology and drug development.

METHODS (2022)

Article Chemistry, Medicinal

Oxidation and Deamidation of Monoclonal Antibody Products: Potential Impact on Stability, Biological Activity, and Efficacy

Surbhi Gupta et al.

Summary: Therapeutic proteins, especially monoclonal antibodies (mAbs), play a significant role in human health. The physical and chemical degradation, particularly oxidation and deamidation, can affect the properties and efficacy of the products.

JOURNAL OF PHARMACEUTICAL SCIENCES (2022)

Article Biochemical Research Methods

Absolute Quantitation of N-Nitrosamines by Coulometric Mass Spectrometry without Using Standards

Qi Wang et al.

Summary: This study describes a novel absolute quantitation strategy for N-nitrosamines using coulometric mass spectrometry (CMS) without the need for standards. By converting N-nitrosamines into electrochemically active hydrazine and quantifying it using CMS, this method allows for the accurate quantification of N-nitrosamines in drugs with complex structures.

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY (2022)

Article Biochemical Research Methods

Coulometry-assisted quantitation in spray ionization mass spectrometry

Xiaowei Song et al.

Summary: Quantification of target analyte in a mixture can be achieved by combining coulometric measurements with spray ionization mass spectrometry. Electrochemical oxidation potential is optimized with cyclic voltammetry, followed by spray ionization for measuring oxidation yield. The method does not require a standard for constructing the quantitation curve and demonstrates an accuracy of around 15% for various species.

JOURNAL OF MASS SPECTROMETRY (2021)

Article Biochemical Research Methods

Quantitative Proteomic Analysis Revealed Broad Roles of N6-Methyladenosine in Heat Shock Response

Weili Miao et al.

Summary: The study utilized SILAC and LC-MS/MS techniques to identify significant alterations in protein expression levels in human cells in response to heat shock, resulting in both decreased and increased protein expressions. Using targeted proteomics, the research further examined changes in kinase proteins, revealing that several kinases showed significant changes after heat shock.

JOURNAL OF PROTEOME RESEARCH (2021)

Article Biochemical Research Methods

Absolute Quantitation of Tryptophan-Containing Peptides and Amyloid β-Peptide Fragments by Coulometric Mass Spectrometry

Yongling Ai et al.

Summary: The article presents a novel CMS method for absolute quantitation of peptides based on tryptophan electrochemical oxidation, eliminating the need for isotope-labeled internal standards. The method demonstrates successful quantitation of tryptophan-containing peptides as well as proteins and Aβ peptide fragments. The CMS approach shows promise in advancing proteomics research by providing an alternative to costly and time-consuming isotope-labeled peptide syntheses.

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY (2021)

Article Chemistry, Analytical

Absolute Quantitation of Proteins by Coulometric Mass Spectrometry

Pengyi Zhao et al.

ANALYTICAL CHEMISTRY (2020)

Article Biotechnology & Applied Microbiology

Purification of GST-Fused Cyanobacterial Central Oscillator Protein KaiC

P. Kim et al.

APPLIED BIOCHEMISTRY AND MICROBIOLOGY (2020)

Article Biochemical Research Methods

Influence of the amino acid composition on the ionization efficiencies of small peptides

Piia Liigand et al.

JOURNAL OF MASS SPECTROMETRY (2019)

Article Chemistry, Analytical

High-Throughput Targeted Quantitative Analysis of the Interaction between HSP90 and Kinases

Weili Miao et al.

ANALYTICAL CHEMISTRY (2019)

Article Physics, Atomic, Molecular & Chemical

Improvements for absolute quantitation using electrochemical mass spectrometry

Pengyi Zhao et al.

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY (2019)

Article Biochemical Research Methods

Absolute Quantitation of Oxidizable Peptides by Coulometric Mass Spectrometry

Pengyi Zhao et al.

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY (2019)

Article Biochemical Research Methods

A New Quantification Method Using Electrochemical Mass Spectrometry

Chang Xu et al.

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY (2019)

Article Chemistry, Analytical

Relative and Absolute Quantitation in Mass Spectrometry-Based Proteomics

J. Astor Ankney et al.

ANNUAL REVIEW OF ANALYTICAL CHEMISTRY, VOL 11 (2018)

Review Biotechnology & Applied Microbiology

Challenges to industrial mAb bioprocessing - removal of host cell proteins in CHO cell bioprocesses

Sarah Gilgunn et al.

CURRENT OPINION IN CHEMICAL ENGINEERING (2018)

Article Biotechnology & Applied Microbiology

Comparison of platform host cell protein ELISA to process-specific host cell protein ELISA

Feny Gunawan et al.

BIOTECHNOLOGY AND BIOENGINEERING (2018)

Review Biochemical Research Methods

Monitoring proteolytic processing events by quantitative mass spectrometry

Mariel Coradin et al.

EXPERT REVIEW OF PROTEOMICS (2017)

Article Biochemical Research Methods

Peptide Selection for Targeted Protein Quantitation

Cristina Chiva et al.

JOURNAL OF PROTEOME RESEARCH (2017)

Article Chemistry, Analytical

A Novel Sample Preparation for Shotgun Proteomics Characterization of HCPs in Antibodies

Lihua Huang et al.

ANALYTICAL CHEMISTRY (2017)

Review Biotechnology & Applied Microbiology

Protein deamidation in biopharmaceutical manufacture: understanding, control and impact

David Gervais

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY (2016)

Review Biochemical Research Methods

Host cell protein analysis in therapeutic protein bioprocessing - methods and applications

Anne Luise Tscheliessnig et al.

BIOTECHNOLOGY JOURNAL (2013)

Correction Multidisciplinary Sciences

Global quantification of mammalian gene expression control (vol 473, pg 337, 2011)

Bjoern Schwanhaeusser et al.

NATURE (2013)

Article Multidisciplinary Sciences

Molecular Ageing of Alpha- and Beta-Synucleins: Protein Damage and Repair Mechanisms

Vasanthy Vigneswara et al.

PLOS ONE (2013)

Article Ophthalmology

Age-Dependent Deamidation of Lifelong Proteins in the Human Lens

Peter G. Hains et al.

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE (2010)

Review Biochemical Research Methods

Isotope dilution strategies for absolute quantitative proteomics

Virginie Brun et al.

JOURNAL OF PROTEOMICS (2009)

Article Biochemistry & Molecular Biology

Post-translational modifications of recombinant proteins: Significance for biopharmaceuticals

Nigel Jenkins et al.

MOLECULAR BIOTECHNOLOGY (2008)