4.8 Article

Deeper Understanding of Solvent-Based Ambient Ionization Mass Spectrometry: Are Molecular Profiles Primarily Dictated by Extraction Mechanisms?

Journal

ANALYTICAL CHEMISTRY
Volume 94, Issue 42, Pages 14734-14744

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.2c03360

Keywords

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Funding

  1. Welch Foundation
  2. [F-1895-20190330]
  3. [F-1155]

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In this study, solvent-based ambient ionization mass spectrometry techniques were used to investigate the extraction processes of lipids in biological tissues. The results suggest that the physicochemical properties of the solvent systems and the extraction time significantly influence the molecular extraction and detection of lipids.
Solvent-based ambient ionization mass spectrometry (MS) techniques provide a powerful approach for direct chemical analysis and molecular profiling of biological tissues. While molecular profiling of tissues has been widely used for disease diagnosis, little is understood about how the interplay among solvent properties, matrix effects, and ion suppression can influence the detection of biological molecules. Here, we perform a systematic investigation of the extraction processes of lipids using an ambient ionization droplet microsampling platform to investigate how the physicochemical properties of the solvent systems and extraction time influence molecular extraction and detection. Direct molecular profiling and quantitative liquid chromatography-mass spectrometry (LC-MS) of discrete solvent droplets after surface sampling were investigated to provide insights into extraction and ionization mechanisms. The results of this study suggest that intermolecular interactions such as hydrogen bonding play a major role in extraction and detection of lipids using solvent-based ambient ionization techniques. In addition, extraction time was observed to impact the molecular profiles obtained, suggesting optimization of this parameter can be performed to favor detection of specific analytes.

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