Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 414, Issue 23, Pages 6977-6987Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-022-04266-2
Keywords
Double-emulsion droplets; Electrospray ionization mass spectrometry; Microfluidics; Lipase-catalyzed reaction
Funding
- Deutsche Forschungsgemeinschaft [251124697, FOR 2177 In-Chem]
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Microfluidic double-emulsion droplets enable the realization and study of biphasic chemical processes at the nanoliter scale. In this study, double emulsions of a rare type were used to catalyze a lipase-catalyzed reaction, allowing real-time MS analysis. The formation of hydrophilic reaction products within the double-emulsion droplets was successfully verified.
Microfluidic double-emulsion droplets allow the realization and study of biphasic chemical processes such as chemical reactions or extractions on the nanoliter scale. Double emulsions of the rare type (o(1)/w/o(2)) are used here to realize a lipase-catalyzed reaction in the non-polar phase. The surrounding aqueous phase induces the transfer of the hydrophilic product from the core oil phase, allowing on-the-fly MS analysis in single double droplets. A microfluidic two-step emulsification process is developed to generate the (o(1)/w/o(2)) double-emulsion droplets. In this first example of microfluidic double-emulsion MS coupling, we show in proof-of-concept experiments that the chemical composition of the water layer can be read online using ESI-MS. Double-emulsion droplets were further employed as two-phase micro-reactors for the hydrolysis of the lipophilic ester p-nitrophenyl palmitate catalyzed by the Candida antarctica lipase B (CalB). Finally, the formation of the hydrophilic reaction product p-nitrophenol within the double-emulsion droplet micro-reactors is verified by subjecting the double-emulsion droplets to online ESI-MS analysis.
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