4.7 Article

CoN4-supported Co2N metal clusters for developing sensitive chemiluminescent immunochromatographic assays

Journal

ANALYTICA CHIMICA ACTA
Volume 1232, Issue -, Pages -

Publisher

ELSEVIER
DOI: 10.1016/j.aca.2022.340478

Keywords

CoN4-supportedCo2N clusters; Synergetic catalysts; Chemiluminescence enhancement; Fenton-like activity; Immunochromatographic assays

Funding

  1. National Natural Science Foun-dation of China
  2. Natural Science Foundation of Chongqing
  3. Fundamental Research Funds for the Central Universities
  4. [21804111]
  5. [22074123]
  6. [21775125]
  7. [cstc2021jcyj-msxmX0248]
  8. [SWU120077]

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Single-atom site catalysts with optimal catalytic efficiency are widely used in various fields. However, the low loading ratio of active sites hampers their catalytic activity improvement. Here, a low-temperature reduction method was employed to synthesize CoN4-supported Co2N clusters, which exhibited superior chemiluminescent catalytic capacity compared to pure single-atom site catalysts.
In view of the optimal catalytic efficiency (-100%), single-atom site catalysts are being widely exploited in a range of areas including organic synthesis, energy conversion, environmental remediation, biotherapy, etc. However, low loading ratio of the unitary active sites on single-atom site catalysts dramatically hinders the remarkable improvement of their catalytic activity. Hereby, a facile low-temperature reduction protocol was adopted for synthesizing CoN4-supported Co2N metal clusters on graphitic carbon nitride, which show the remarkably superior chemiluminescent (CL) catalytic capacity than some reported pure single-atom site cata-lysts. Nitrogen-encapsulated Co2N clusters coupled with isolated Co-N4 moieties (Co2N@Co-N4) endowed the synergetic catalysts with high Co content of 53.2 wt%. Through X-ray absorption spectroscopy, the synergetic active sites (Co2N@Co-N4) afforded the CoN4-supported Co2N clusters with the remarkable catalytic activity for accelerating the decomposition of H2O2 to produce extensive superoxide radical anion rather than singlet oxygen or hydroxyl radical. Therefore, the CoN4-supported Co2N clusters possessed the superb enhancement effect on luminol-H2O2 CL reaction by-22829 times. The CoN4-supported Co2N clusters were utilized as signal probes to establish a CL immunochromatographic assay (ICA) platform for quantitating mycotoxins. Herein, aflatoxin B1 was employed as a mode analyte and the limit of detection was as low as 0.33 pg mL-1 (3 sigma). As a proof-of -principle work, the developed ICA protocol was successfully employed on the detection of aflatoxin B1 spiked in Angelica dahurica and Ganoderma lucidum with acceptable recoveries of 84.0-107.0%. The ideal practicability of the work elucidates that CoN4-supported Co2N clusters showed a new perspective for developing the sensitive CL biosensing.

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