Quantitative Analysis of the Histological Features of Tuberous Breast

Introduction Tuberous breast deformity (TB) is a condition mostly characterized by breast stenosis, areolar widening and glandular asymmetry. The most accredited hypothesis describes an abnormal thickening of the fascia corporis that might influence an alteration in the glandular development, limiting the horizontal growth of breast parenchyma. Alterations in the extracellular matrix components (ECM) might be involved in the abnormal breast development. Patients The aim of our case control study is to use histological specimens to analyze qualitative and quantitative differences in collagen fibers, elastic fibers and vessel densities in TB and normal breasts of 20 patients using a software for digital pathology. Results The quantitative findings showed increasing concentrations of collagen fibers and decreasing elastic fibers in TB, compared to normal breasts. No difference was seen in vessel density among the two groups. The qualitative findings highlighted differences in the distribution of the ECM among the TB specimens. Collagen fibers showed a packed appearance rather a scattered distribution, while elastic fibers visibly presented a reduction and a focal distribution of their concentration. Conclusions The study proposes a correlation between abnormalities in ECM concentrations and TB, resulting in a higher degree of fibrosis and in the characteristic stenotic and less elastic morphology of the deformity.

Automated summary

This study used histological specimens and digital pathology software to analyze the differences in collagen fibers and elastic fibers in patients with tuberous breast deformity (TB) compared to normal breasts. The results showed increased concentrations of collagen fibers and decreased elastic fibers in TB, highlighting the correlation between abnormal extracellular matrix concentrations and the characteristic stenotic and less elastic morphology of the deformity.