Green versatile micellar electrokinetic chromatographic method for determination of six antimicrobial and anti-inflammatory drugs in combined dosage forms

A fast reliable micellar electrokinetic methodology was investigated for the concurrent quantitation of six antimicrobial and anti-inflammatory drugs, namely, ciprofloxacin, dexamethasone, metronidazole, ornidazole, spiramycin and tinidazole. The method has the merits of rapidity, precision, and sensitivity. The separation was carried out in less than 7 min by applying a basic background electrolyte consisting of 25 mM disodium tetraborate buffer, pH 9 containing 50 mM SDS at 25 kV using photodiode array detector at 230 and 315 nm. The internal standard used during analysis was cromolyn sodium and validation was carried out following ICH guidelines. The proposed method showed linear response over the range from 0.5 to 10.0 & mu;g mL-1 reaching limits of detection and limits of quantitation in the ranges of 0.09-0.2 & mu;g mL-1 and 0. 27-0.6 respectively. The method's greenness was estimated using the GAPI tool where excellent greenness was concluded. Co-formulated or single-ingredient commercial preparations were investigated and the results were statistically evaluated.

Automated summary

A fast reliable micellar electrokinetic methodology was developed for the quantitation of six antimicrobial and anti-inflammatory drugs. The method showed rapidity, precision, and sensitivity, with a separation time of less than 7 minutes. The proposed method had a linear response range, detection limits, and quantitation limits suitable for analysis. The method's greenness was also evaluated and found to be excellent. Commercial preparations were investigated and statistically evaluated.