4.8 Article

Breaching Bacterial Biofilm Barriers: Efficient Combinatorial Theranostics for Multidrug-Resistant Bacterial Biofilms with a Novel Penetration-Enhanced AIEgen Probe

Journal

ACS APPLIED MATERIALS & INTERFACES
Volume 14, Issue 37, Pages 41671-41683

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/acsami.2c07378

Keywords

antibacterial; multidrug-resistant bacterial biofilm; theranostics; ultrafast identification; AIEgen probe

Funding

  1. National Natural Science Foundation of China [82272067, 81971678, 81671756, 22107123]
  2. Innovation Fund for Post graduate Students of Hunan Province [CX20200273]
  3. Innovation Fund for Post graduate Students of Central South University [2020zzts241]

Ask authors/readers for more resources

A water-soluble probe with membrane-active fluorescence and broad-spectrum antimicrobial actions was designed to inhibit and eradicate microbial biofilms effectively, especially against methicillin-resistant Staphylococcus atoms (MRSA). The probe demonstrated strong antimicrobial effects comparable to vancomycin and effectively inhibited biofilm formation without toxicity to mammalian cells. It could penetrate through biofilm barriers and disrupt existing biofilms, providing a novel theranostic strategy for the treatment of multidrug-resistant bacterial infections and antibiofilm medications.
The formation of microbial biofilms is acknowledged as a major virulence factor in a range of persistent local infections. Failures to remove biofilms with antibiotics foster the emergence of antibiotic-resistant bacteria and result in chronic infections. As a result, the construction of effective biofilm-inhibiting and biofilm-eradicating chemicals is urgently required. Herein, we designed a water-soluble probe APDIS for membrane-active fluorescence and broad-spectrum antimicrobial actions, particularly against methicillin-resistant Staphylococcus atoms (MRSA), which shows multidrug resistance. In vitro and in vivo experiments demonstrate its high antibacterial effects comparable to vancomycin. Furthermore, it inhibits biofilm formation by effectively killing planktonic bacteria at low inhibitory concentrations, without toxicity to mammalian cells. More importantly, this probe can efficiently penetrate through biofilm barriers and exterminate bacteria that are enclosed within biofilms and startle existing biofilms. In the mouse model of implant-related biofilm infections, this probe exhibits strong antibiofilm activity against MRSA biofilms, thus providing a novel theranostic strategy to disrupt biofilms in vivo effectively. Our results indicate that this probe has the potential to be used for the development of a combinatorial theranostic platform with synergistic enhanced effects for the treatment of multidrug-resistant bacterial infections and antibiofilm medications.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.8
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available