Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 14, Issue 45, Pages 51234-51243Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.2c16267
Keywords
multifunctional nanomaterials; dual-signal readout; synergistic effect; signal amplification; food safety
Funding
- Laboratory of Lingnan Modern Agriculture Project [NZ2021036]
- National Natural Science Foundation of China [32172293]
- Wuhan Science and Technology Planning Project [2020030603012347]
- Key R&D Program of Hubei Province [2020BBA053]
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In this study, a dual-signal readout biosensing platform was developed using a new nanomaterial. The platform utilized Pt nanoparticles and horseradish peroxidase to enhance the colorimetric signal brightness, while also protecting the enzyme activity. The synergistic catalytic effect of Pt nanoparticles and HRP improved the sensitivity of detection. The biosensor displayed high sensitivity and showed potential for analyzing various targets.
To address the urgent demand for sensitive and stable detection applications, significant efforts have been made in the development of dual-signal readout assays for precise target detection and timely health risk control. Here, a new nanomaterial, Pt@PCN-224-HRP-initiator DNA (PP-HRP-iDNA), was exploited to construct a dual-signal readout biosensing platform. Zr-MOF (PCN-224) was loaded with as many Pt nanoparticles (NPs) and as much horseradish peroxidase (HRP) as possible to enhance the brightness of the colorimetric signal recognizable to the naked eye while also acting as a gatekeeper to protect the enzyme activity and ensuring the stability of the assay process. Moreover, the Pt NPs and HRP displayed a synergistic catalytic effect, which promoted the sensitivity of detection. Further, the formation of the Zr-O-P bond eliminated the instability of the interactions between PCN-224 and iDNA in a controllable manner. After the immunoreaction, iDNA stimulated a hybridization chain reaction, resulting in a significant reduction of the fluorescent DNA in the supernatant and a fluorescent signal change. Subsequently, the PP-HRP-iDNA probe implemented UV-light response (450 nm) where 3,3 ',5,5 '-tetramethylbenzidine was used as a substrate for the colorimetric signal readout. By virtue of the nanomaterial-modulated transduction mechanism and the antigen-antibody interactions, this dual-signal biosensor displays high sensitivity, with a limit of detection of 0.65 pg/mL for aflatoxin B1 and 4 CFU/ mL for Salmonella enteritidis, suggesting the detection potential of the biosensing platform for analyzing various targets.
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