Journal
JOURNAL OF GENERAL VIROLOGY
Volume 97, Issue -, Pages 1551-1556Publisher
MICROBIOLOGY SOC
DOI: 10.1099/jgv.0.000486
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Funding
- National Basic Research Project [2012CB518904]
- National Natural Science Foundation of China [81101243, 31270974]
- National Major Special Program of Science and Technology of China [2008ZX10004-015, 2013ZX10004001]
- Excellent Young Scientist Program from the National Natural Science Foundation of China [81522025]
- UK Academy of Medical Sciences
- NSFC [81661130162]
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Duck Tembusu virus (DTMUV), a newly identified flavivirus, has rapidly spread to China, Malaysia and Thailand. The potential threats to public health have been well-highlighted; however its virulence and pathogenesis remain largely unknown. Here, by using reverse genetics, a recombinant chimeric DTMUV based on Japanese encephalitis live vaccine strain SA14-14-2 was obtained by substituting the corresponding prM and E genes (named ChinDTMUV). In vitro characterization demonstrated that ChinDTMUV replicated efficiently in mammalian cells with small-plaque phenotype in comparison with its parental viruses. Mouse tests showed ChinDTMUV exhibited avirulent phenotype in terms of neuroinvasiveness, while it retained neurovirulence from its parental virus DTMUV. Furthermore, immunization with ChinDTMUV was evidenced to elicit robust IgG and neutralizing antibody responses in mice. Overall, we successfully developed a viable chimeric DTMUV, and these results provide a useful platform for further investigation of the pathogenesis of DTMUV and development of a live attenuated DTMUV vaccine candidate.
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