Journal
JOURNAL OF GENERAL VIROLOGY
Volume 97, Issue -, Pages 1458-1461Publisher
MICROBIOLOGY SOC
DOI: 10.1099/jgv.0.000453
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Funding
- Foundation for University Key Teacher by the Henan Province, China [2014GGJS-037]
- Henan Key Laboratory of Fruit and Cucurbit Biology Foundation [HNS-201508-9]
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Cucurbit chlorotic yellows virus (CCYV), a recently identified bipartite crinivirus, causes economic losses in cucurbit plants. CCYV is naturally transmitted only by whitefly Bemisia tabaci. Here we constructed full-length cDNA clones of CCYV (RNA1 and RNA2) fused to the T7 RNA polymerase promoter and the cauliflower mosaic virus 35S promoter. CCYV replicated and accumulated efficiently in Cucumis sativus protoplasts transfected with in vitro transcripts. Without RNA2, RNA1 replicated efficiently in C. sativus protoplasts. Agroinoculation with the infectious cDNA clones of CCYV resulted in systemic infection in the host plants of C. sativus and Nicotiana benthamiana. Virus derived from the infectious clones could be transmitted between cucumber plants by vector whiteflies. This system will greatly enhance the reverse genetic studies of CCYV gene functions.
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