Journal
JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
Volume 31, Issue 8, Pages 1429-1435Publisher
WILEY-BLACKWELL
DOI: 10.1111/jgh.13300
Keywords
gastric cancer; miRNA; young adults
Categories
Funding
- Korea Health Industry Development Institute (KHIDI)
- Ministry of Health and Welfare, Republic of Korea [HI06C0868]
- Bio and Medical Technology Development Program of the National Research Foundation (NRF) - Korean government (MEST) [2011-0019632]
- Asan Institute for Life Sciences [2015-7402, 2015-653]
- National Research Foundation of Korea [2011-0019632] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Ask authors/readers for more resources
Background and AimIt is generally assumed that gastric cancer (GC) in young patients has different clinicopathologic characteristics than that of elderly patients. Although recurrence is an important factor in determining prognosis, traditional clinicopathological factors are sometimes inadequate for predicting recurrence in individuals. Therefore, we aimed to identify miRNAs with the potential to predict recurrence in young patients. MethodsYoung patients (age <40years) undergoing gastrectomy for potentially curable GC (stage III) at Asan Medical Center participated in this study. A total of 50 GC patients with (n=28) and without (n=22) GC recurrence were selected for analysis. miRNA microarrays were analyzed to screen tissue samples (n=7), and we validated the selected miRNAs by quantitative PCR in validation samples (n=43). ResultsTwo miRNAs (hsa-miR-21-5p and hsa-miR-451a) identified in the microarray analysis were evaluated in the validation samples. Among the validation samples containing intratumoral stroma70 (n=35), hsa-miR-21-5p was more highly expressed in the recurrence group than in the nonrecurrence group (fold change 1.82, P=0.03). In the validation samples with intratumoral stroma70, the Ct of hsa-miR-21-5p, which was >3.35, had a sensitivity and specificity of 86.7% and 65.5%, respectively, for predicting recurrence, with an area under the ROC curve of 0.723. ConclusionsmiR-21-5p may be useful as a predictor of recurrence in young GC patients whose tumors contain a high proportion of intratumoral stroma. The combination of this miRNA with conventional clinicopathological factors should allow patient prognoses to be more accurately predicted.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available