Journal
BIOPHYSICAL REVIEWS
Volume 14, Issue 4, Pages 1051-1065Publisher
SPRINGERNATURE
DOI: 10.1007/s12551-022-00989-9
Keywords
Retinal pigment epithelium; Lipofuscin granules; Bisretinoids; Photosensitization; Autofluorescence; AMD
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Funding
- Russian Science Foundation [22-24-00549]
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Lipofuscin granules (LGs) accumulate in the retinal pigment epithelium (RPE) cells and can cause pathology and accelerate aging. The review examines the composition, spectral properties, and photoactivity of LGs. Studies show that the fluorescent characteristics of LGs change in pathology and after exposure to ionizing radiation. Improved methods of early diagnosis of degenerative diseases can be developed by analyzing the fluorescence spectra and kinetics of oxidized products of LG fluorophores.
Lipofuscin granules (LGs) are accumulated in the retinal pigment epithelium (RPE) cells. The progressive LG accumulation can somehow lead to pathology and accelerate the aging process. The review examines composition, spectral properties and photoactivity of LGs isolated from the human cadaver eyes. By use of atomic force microscopy and near-field microscopy, we have revealed the fluorescent heterogeneity of LGs. We have discovered the generation of reactive oxygen species by LGs, and found that LGs and melanolipofuscin granules are capable of photoinduced oxidation of lipids. It was shown that A2E, as the main fluorophore (bisretinoid) of LGs, is much less active as an oxidation photosensitizer than other fluorophores (bisretinoids) of LGs. Photooxidized products of bisretinoids pose a much greater danger to the cell than non-oxidized one. Our studies of the fluorescent properties of LGs and their fluorophores (bisretinoids) showed for the first time that their spectral characteristics change (shift to the short-wavelength region) in pathology and after exposure to ionizing radiation. By recording the fluorescence spectra and fluorescence decay kinetics of oxidized products of LG fluorophores, it is possible to improve the methods of early diagnosis of degenerative diseases. Lipofuscin (aging pigment) is not an inert slag. The photoactivity of LGs can pose a significant danger to the RPE cells. Fluorescence characteristics of LGs are a tool to detect early stages of degeneration in the retina and RPE.
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