Journal
JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
Volume 31, Issue 2, Pages 384-393Publisher
WILEY
DOI: 10.1111/jgh.13180
Keywords
esophageal squamous cell carcinoma; LASP1; microRNA-1; TAGLN2
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ObjectiveThis study determined the expression of microRNA-1 in esophageal squamous cell carcinoma (ESCC) tissue and cell lines to evaluate its effects on clinicopathological parameters and its target genes LASP1 and TAGLN2. MethodsThe expression of miR-1, lasp1, and tagln2 was detected in 55 ESCC tissues and adjacent normal tissues by reverse transcription-polymerase chain reaction (RT-PCR). The association between miR-1, lasp1, and tagln2 expression and clinicopathological characteristics was observed. MicroRNA-1 (mimics-miR-1) and its inhibitor (Inhibitor-miR-1) were transfected into esophageal cancer cells KYSE 510 and Eca 109; cell proliferation, migration, and invasion assays were carried out. Plasmid construction and dual-luciferase reporter assay were also carried out to indicate whether LASP1 and TAGLN2 were miR-1 target genes. The expression of LASP1 and TAGLN2 was detected with Western blot methods in cell lines, by immunohistochemistry in ESCC tissue. ResultsThe gene expression level of microRNA-1 in cancer tissues was significantly lower than that in adjacent normal tissues (P<0.01). The expression of miR-1 in ESCC was correlated with involvement of lymph nodes (P=0.002), histologic classification (P=0.000), and vessel invasion (P=0.022). The expression of lasp1 and tagln2 increased in cancer tissues compared with in adjacent normal tissues (P<0.05). MiR-1 suppresses the cell growth, migration, and invasion in vitro. The expression of LASP1 and TAGLN2 decreased in mimics-miR-1 transfected cells, and increased in inhibitor-miR-1 transfected cells. Luciferase reporter assay confirmed that LASP1 and TAGLN2 mRNA actually had the target sites of miR-1. ConclusionsmiR-1 suppresses cell proliferation, invasiveness, metastasis, and progression of ESCC by binding its targeted genes LASP1 and TAGLN2.
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