Journal
ADVANCED FUNCTIONAL MATERIALS
Volume 25, Issue 28, Pages 4412-4418Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adfm.201501668
Keywords
calmodulin; conformation changes; cationic-conjugated polymers; graphene oxide
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Funding
- Scientific Innovation Grant for Excellent Young Scientists of Hebei University of Technology [2013003]
- 100 Talents Program of Hebei Province, China [E2014100004]
- Program for Excellent Innovative Talents in Universities of Hebei Province, China [BJ2014011]
- Hebei Province Science Foundation [QN2014024, B2015202330]
- National Natural Science Foundation of China [21373243]
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The conformation of calmodulin (CaM) changes from closed configuration to open one, converting to a claviform dumbbell-shaped biomolecule upon Ca2+-binding. A hybrid probe of graphene oxide (GO) cationic conjugated polymer for detection of the conformation transition of CaM by using FRET technique is demonstrated. The stronger hydrophobic interaction and weaker electrostatic repulsion leads to more CaM adsorption to the surface of GO upon binding with Ca2+ than that of CaM in the absence of Ca2+ (apoCaM), resulting in much farther proximity between poly[(9,9-bis(6-N,N,N-trimethylammonium)hexyl)-fluorenylene phenylene dibromide] (PFP) and green fluorescent protein labeled at the N-terminus of CaM and therefore much weaker FRET efficiency for PFP/Ca2+/CaM in comparison with that of PFP/apoCaM in the presence of GO. Notably, the assembly of CaM with GO is quantitatively and reversibly controlled by Ca2+ ions.
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