Journal
JOURNAL OF FOOD ENGINEERING
Volume 178, Issue -, Pages 39-46Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.jfoodeng.2016.01.005
Keywords
AFM; Dairy powders; Surface characterization; ToF-SIMS; WPI
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Dairy powders are stable ingredients that are frequently stored prior to use. In fact, during powder manufacture, precautionary measures are taken to ensure optimal technological and nutritional functionalities, but during storage, changes in physico-chemical and functional properties of whey protein isolate (WPI) powders were extensively outlined. In the present study, WPI powders were stored at 60 degrees C and 0.2 water activity for one month and local modifications on particle surface were explored. Atomic force microscopy (AFM), specifically in chemical force microscopy (CFM) mode, was used to follow surface modifications during storage. An increase in surface hydrophobicity was noticed when powders were stored at high temperatures. Complementary techniques such as microscopy and Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS) showed a crackled surface and a decrease in the presence of amino acid at surface. Finally, powder browning and fluorescence of components on particle surface suggested that Maillard reaction is the main phenomenon responsible for powder alteration during storage. (C) 2016 Elsevier Ltd. All rights reserved.
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