Journal
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
Volume 47, Issue -, Pages 101-112Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jfca.2016.01.005
Keywords
Carotenoids; Isomers; HPLC; C-18 and C-30 stationary phases; Retention time; Epoxide; kappa-Carotenoids; Polarity; Geometrical isomers; Food analysis
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Funding
- Hungarian Scientific Research Fund [OTKA 115931]
- PTE AOK [KA-2015-18]
- Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences
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We provide a database which presents the separation characteristics of C-18 and C-30 stationary phases using carotenoids with diverse structures, including isomers, from the apolar carotenes to the very polar tetrahydroxy xantophylls. The retention behavior of more than 90 carotenoids on a C-18 and C-30 stationary phase were studied. The influence of structural differences of the carotenoids on the elution order for the two columns is discussed. Octadecyl bonded silica phase produces good separation for the whole polarity range of carotenoids. On this phase carotenoids are eluted in the order of their polarity. The examined polar compounds, including optical isomers, could be well separated on a C-18 phase. However, the separation of regio- and geometrical isomers of carotenoids, as well as non-polar carotenes, is better achieved using a C-30 phase. (C) 2016 Elsevier Inc. All rights reserved.
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