Journal
ANATOMY & CELL BIOLOGY
Volume 55, Issue 2, Pages 229-238Publisher
MEDRANG
DOI: 10.5115/acb.21.233
Keywords
Extracellular matrix; Tissue engineering; Mesentery; Tenascin-C; Cell migration
Categories
Funding
- Ferdowsi University of Mashhad
- Kavian Institute of Higher Education
Ask authors/readers for more resources
In this study, a cell migration model was established to observe the polarity and migration of blastema cells by decellularizing bovine mesenteric tissue. The results showed that this three-dimensional model of cellular migration can be used to study cell behaviors in vitro.
Cell migration is an essential process in embryonic development, wound healing, and pathological conditions. Our knowledge of cell migration is often based on the two dimentional evaluation of cell movement, which usually differs from what occurred in vivo. In this study, we investigated cellular migration from blastema tissue toward bovine decellularized mesentery tissue. In this regard, fibronectin (FN) was assessed to confirm cell migration. Therefore, we established a cell migration model using blastema cells migration toward the extracellular matrix derived from bovine mesenteric tissue. A physiochemical decellularization method was utilized based on freeze-thaw cycles and agitation in sodium dodecyl sulfate and Triton X-100 to remove cells from the extracellular matrix (ECM) of bovine mesenteric tissue. These types of matrices were assembled by the rings of blastema tissues originated from the of New Zealand rabbits pinna and cultured in a medium containing FN in different days in vitro, and then they are histologically evaluated, and the expression of the Tenascin C gene is analyzed. By means of tissue staining and after confirmation of the cell removal from mesenteric tissue, polarity, and migration of blastema cells was observed in the interaction site with this matrix. Also, the expression of the Tenascin C gene was assessed on days 15 and 21 following the cell culture process. The results showed that the three dimentional model of cellular migration of blastema cells along with the ECM could be a suitable model for investigating cell behaviors, such as polarity and cell migration in vitro.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available