Journal
HELIYON
Volume 8, Issue 6, Pages -Publisher
CELL PRESS
DOI: 10.1016/j.heliyon.2022.e09660
Keywords
TBrC; SARS-CoV-2; M-pro/3CL; ACE2; Mutant spike proteins; NF-?B
Categories
Funding
- Shandong Provincial Natural Science Foundation [ZR2019MH076, 20092009GG10002087]
- National Natural Science Foundation of China [81603024, 30973553]
- National Key Research and Develop-ment Program of China [2017YFB0702600, 2017YFB0702602, 2017YFB0702602-2]
- Ministry of Science and Technology of the People's Republic of China [2012AA020206]
- Science and Technology Support Program for Youth Innovation in Universities of Shandong [2020KJM003]
- Corbett Estate Fund for Cancer Research [62285-531021-41800, 62285-531021-51800, 62285-531021-61800, 62285-531021-71800]
- Corbett Estate Fund for Cancer Research
- Taishan Scholar Project
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Recent research has discovered that L-theanine from tea and its derivative TBrC have potential inhibitory activities against SARS-CoV-2, as well as anti-tumor effects on lung cancer cells without affecting normal lung cells. This suggests a potential protective effect of TBrC and L-theanine against pulmonary damage in patients infected with SARS-CoV-2, particularly lung cancer patients.
Recent research efforts have shown that tea has activities against SARS-CoV-2. However, the active compounds and the action mechanisms are largely unknown. Here we study the inhibitory potential of L-theanine from tea and its semi-synthesized derivative, a small-molecule fluorescent compound, ethyl 6-bromocoumarin-3-carboxylyl L-theanine (TBrC) against infection and replication of SARS-CoV-2 and the underlying mechanisms of action. We reveal that TBrC has potential activities against SARS-CoV-2 in addition to its activity against lung cancer. TBrC showed extracellular inhibition of SARS-CoV-2 Mpro/3CL and the host cell receptor ACE2 while interacting with the viral spike glycoproteins (wild-type, Delta, and Omicron mutants). Moreover, TBrC and L-theanine significantly suppressed growth and TNF alpha-induced nuclear transcriptional activation of NF-kappa B in human lung cancer cells without affecting the viability of normal lung cells, suggesting a potential protection of TBrC and L-theanine from pulmonary damages in SARS-CoV-2 infected patients, especially for lung cancer patients with SARS-CoV-2 infection.
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