Journal
ENVIRONMENTAL MICROBIOME
Volume 17, Issue 1, Pages -Publisher
BMC
DOI: 10.1186/s40793-022-00432-2
Keywords
Nitritation; Anammox; Ammonia; Nitrite; Brocadia; Nitrosomonas; Nitric oxide
Categories
Funding
- Danish Agency for Science Technology and Innovation
- Research Project LaGas [12-132633]
- Research Centre EcoDesign [09-067230]
- EU-FP7 People: Marie-Curie Actions [REA 607492]
- Villlum Fonden [Expa-N 13391]
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This study analyzed the microbiome of an efficient autotrophic ammonia removal process and found that sequential feeding and periodic aeration can achieve high efficiency. The microbiome consisted of abundant aerobic and anaerobic ammonia oxidizing bacteria and scarce nitrite oxidizing bacteria. Additionally, there was a mutual dependency in amino acid and vitamin synthesis between autotrophic and heterotrophic community members.
Obtaining efficient autotrophic ammonia removal (aka partial nitritation-anammox, or PNA) requires a balanced microbiome with abundant aerobic and anaerobic ammonia oxidizing bacteria and scarce nitrite oxidizing bacteria. Here, we analyzed the microbiome of an efficient PNA process that was obtained by sequential feeding and periodic aeration. The genomes of the dominant community members were inferred from metagenomes obtained over a 6 month period. Three Brocadia spp. genomes and three Nitrosomonas spp. genomes dominated the autotrophic community; no NOB genomes were retrieved. Two of the Brocadia spp. genomes lacked the genomic potential for nitrite reduction. A diverse set of heterotrophic genomes was retrieved, each with genomic potential for only a fraction of the denitrification pathway. A mutual dependency in amino acid and vitamin synthesis was noted between autotrophic and heterotrophic community members. Our analysis suggests a highly-reticulated nitrogen cycle in the examined PNA microbiome with nitric oxide exchange between the heterotrophs and the anammox guild.
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