Magnetic Nylon 6 Nanocomposites for the Microextraction of Nucleic Acids from Biological Samples

Magnetic Fe3O4 nanoparticles (MNPs) have great potential for nucleic acid separation, detection, and delivery. MNPs are considered a valuable tool in biomedicine due to their cost-effectiveness, stability, easy surface functionalization, and the possibility of the manipulations under a magnetic field. Herein, the synthesis of magnetic nylon 6 nanocomposites (MNPs@Ny6) was investigated. Transmission electron microscopy (TEM) was used for morphology and size analysis. A new method of UV-induced immobilization of oligonucleotides on MNPs@Ny6 for nucleic acid magnetic separation was proposed. MNPs@Ny6 shows a high oligonucleotide binding capacity of 2.2 nmol/mg with 73.3% loading efficiency. The proposed system has been applied to analyze model mixtures of target RNA on the total yeast RNA background. The RNA target isolation efficiency was 60% with high specificity. The bind RNA release was 88.8% in a quantity of 0.16 nmol/mg. The total RNA capture efficiency was 53%. Considering this, the MNPs@Ny6 is an attractive candidate for nucleic acids-specific magnetic isolation.

Automated summary

Magnetic Fe3O4 nanoparticles have been widely studied for their potential applications in nucleic acid separation, detection, and delivery. In this study, magnetic nylon 6 nanocomposites were synthesized, and a novel method of UV-induced immobilization of oligonucleotides on these nanocomposites for nucleic acid magnetic separation was proposed. The results showed that the synthesized nanocomposites exhibited high oligonucleotide binding capacity and loading efficiency, making them attractive candidates for nucleic acid-specific magnetic isolation.