Ultrasensitive and Selective Detection of Glutathione by Ammonium Carbamate-Gold Platinum Nanoparticles-Based Electrochemical Sensor

Determining the concentration of glutathione is crucial for developing workable medical diagnostic strategies. In this paper, we developed an electrochemical sensor by electrodepositing amino-based reactive groups and gold-platinum nanomaterials on the surface of glassy carbon electrode successively. The sensor was characterized by cyclic voltammetry (CV), field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDX), and electrochemical impedance spectra (EIS). Results showed that Au@Pt nanoparticles with the size of 20-40 nm were presented on the surface of electrode. The sensor exhibits excellent electrocatalytic oxidation towards glutathione. Based on this, we devised an electrochemical biosensor for rapid and sensitive detection of glutathione. After optimizing experimental and operational conditions, a linear response for the concentration of GSH, in the range of 0.1-11 mu mol/L, with low detection and quantification limits of 0.051 mu M (S/N = 3), were obtained. The sensor also exhibits superior selectivity, reproducibility, low cost, as well as simple preparation and can be applied in human serum sample detection.

Automated summary

In this study, an electrochemical sensor for detecting the concentration of glutathione was developed and exhibited excellent performance. The sensor showed high sensitivity, superior selectivity, and reproducibility, making it suitable for human serum sample detection.