Journal
BIOSENSORS-BASEL
Volume 12, Issue 6, Pages -Publisher
MDPI
DOI: 10.3390/bios12060394
Keywords
charge-sensitive optical detection; binding kinetics; label-free; phage-display; peptide; protein
Funding
- National Institutes of Health [R33CA202834, R44GM139535]
- Genentech Inc. [CLL-016354]
Ask authors/readers for more resources
Phage display technology is a useful tool in peptide drug development, but the synthesis of peptides is costly and time-consuming for the accurate measurement of binding kinetics. A sensitive technology is needed to directly measure the binding kinetics of peptides on phages, reducing time and cost.
Phage display technology has been a powerful tool in peptide drug development. However, the supremacy of phage display-based peptide drug discovery is plagued by the follow-up process of peptides synthesis, which is costly and time consuming, but is necessary for the accurate measurement of binding kinetics in order to properly triage the best peptide leads during the affinity maturation stages. A sensitive technology is needed for directly measuring the binding kinetics of peptides on phages to reduce the time and cost of the entire process. Here, we show the capability of a charge-sensitive optical detection (CSOD) method for the direct quantification of binding kinetics of phage-displayed peptides to their target protein, using whole phages. We anticipate CSOD will contribute to streamline the process of phage display-based drug discovery.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available