Fabrication and Characterization of Acute Myocardial Infarction Myoglobin Biomarker Based on Chromium-Doped Zinc Oxide Nanoparticles

In this article, we describe the fabrication and characterization of a sensor for acute myocardial infarction that detects myoglobin biomarkers using chromium (Cr)-doped zinc oxide (ZnO) nanoparticles (NPs). Pure and Cr-doped ZnO NPs (13 x 10(17), 20 x 10(17), and 32 x 10(17) atoms/cm(3) in the solid phase) were synthesized by a facile low-temperature sol-gel method. Synthesized NPs were examined for structure and morphological analysis using various techniques to confirm the successful formation of ZnO NPs. Zeta potential was measured in LB media at a negative value and increased with doping. XPS spectra confirmed the presence of oxygen deficiency in the synthesized material. To fabricate the sensor, synthesized NPs were screen-printed over a pre-fabricated gold-coated working electrode for electrochemical detection of myoglobin (Mb). Cr-doped ZnO NPs doped with 13 x 10(17) Cr atomic/cm(3) revealed the highest sensitivity of similar to 37.97 mu A.cm(-2)nM(-1) and limit of detection (LOD) of 0.15 nM for Mb with a response time of <= 10 ms. The interference study was carried out with cytochrome c (Cyt-c) due to its resemblance with Mb and human serum albumin (HSA) abundance in the blood and displayed distinct oxidation potential and current values for Mb. Cr-doped ZnO NP-based Mb biosensors showed 3 times higher sensitivity as compared to pure ZnO NP-based sensors.

Automated summary

In this article, the fabrication and characterization of a sensor for acute myocardial infarction that detects myoglobin biomarkers using chromium-doped zinc oxide nanoparticles were described. The synthesized nanoparticles showed high sensitivity and a low limit of detection for myoglobin. The chromium-doped zinc oxide nanoparticles-based biosensors exhibited higher sensitivity compared to pure zinc oxide nanoparticles-based sensors.