Journal
BIOSENSORS-BASEL
Volume 12, Issue 7, Pages -Publisher
MDPI
DOI: 10.3390/bios12070446
Keywords
sensor of Fe2+ or Fe3+ ions; non-biofouling layer; poly(2-methyl-2-oxazoline)s; potentiometry; analysis
Funding
- Czech Health Research Council [NU20-06-0024]
- Czech Science Foundation [21-01090S]
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We developed a sensor that can rapidly detect the presence of Fe2+ and/or Fe3+ ions with high selectivity and stability, functioning reliably in a biological environment.
We constructed a sensor for the determination of Fe2+ and/or Fe3+ ions that consists of a polyaniline layer as an ion-to-electron transducer; on top of it, chelating molecules are deposited (which can selectively chelate specific ions) and protected with a non-biofouling poly(2-methyl-2-oxazoline)s layer. We have shown that our potentiometric sensing layers show a rapid response to the presence of Fe2+ or Fe3+ ions, do not experience interference with other ions (such as Cu2+), and work in a biological environment in the presence of bovine serum albumin (as a model serum protein). The sensing layers detect iron ions in the concentration range from 5 nM to 50 mu M.
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