Journal
BIOSENSORS-BASEL
Volume 12, Issue 6, Pages -Publisher
MDPI
DOI: 10.3390/bios12060433
Keywords
immunosensor; HFF-QCMD array; honey; antibiotic; pesticide
Funding
- Ministerio de Economia, Industria y Competitividad de Espana-Agencia Estatal de Investigacion
- FEDER (Fondo Europeo de Desarrollo Regional) [AGL2016-77702-R, AGL2013-48646-R]
- Ministerio de Economia, Industria y Competitividad de Espana [BES-2017-080246]
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This study presents a new method for the simultaneous detection of antibiotics and pesticides in honey. By combining competitive immunoassay with HFF-QCMD array biosensor, this method enables analysis of contaminants in complex food matrices while avoiding elaborate sample pre-treatment, with good sensitivity and short analysis time.
As in the case of the food industry in general, there is a global concern about safety and quality in complex food matrices, such as honey, which is driving the demand for fast, sensitive and affordable analytical techniques across the honey-packaging industry. Although excellent techniques such as liquid chromatography-tandem mass spectrometry (LC-MS/MS) are available, these are located in centralized laboratories and are still lacking in speed, simplicity and cost-effectiveness. Here, a new approach is presented where a competitive immunoassay is combined with a novel High Fundamental Frequency Quartz Crystal Microbalance with Dissipation (HFF-QCMD) array biosensor for the simultaneous detection of antibiotics and pesticides in honey. Concretely, thiabendazole and sulfathiazole residues were monitored in spiked honey samples. Results revealed that HFF-QCMD arrays provide a complementary and reliable tool to LC-MS/MS for the analysis of contaminants in these kinds of complex matrices, while avoiding elaborate sample pre-treatment. The good sensitivity achieved (I-50 values in the 70-720 mu g/kg range) and the short analysis time (60 min for 24 individual assays), together with the ability for multiple analyte detection (24 sensor array) and its cost-effectiveness, pave the way for the implementation of a fast on-line, in situ routine control of potentially hazardous chemical residues in honey.
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