Biosensor Based on Covalent Organic Framework Immobilized Acetylcholinesterase for Ratiometric Detection of Carbaryl

Article Chemistry, Analytical

Two-Dimensional Cobalt-Doped Ti3C2 MXene Nanozyme-Mediated Homogeneous Electrochemical Strategy for Pesticides Assay Based on In Situ Generation of Electroactive Substances

Lei Yu et al.

Summary: In this study, a HEC sensor based on in situ generation of electroactive substances for organophosphate pesticides detection was developed using cobalt-doped 2D nanozymes. The sensor showed high performance and potential application.

ANALYTICAL CHEMISTRY (2022)

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Article Engineering, Environmental

Self-encapsulated enzyme through in-situ growth of polypyrrole for high-performance enzymatic biofuel cell

Jie Huang et al.

Summary: By constructing a conductive enzyme nanocapsule with a protective shell and conductive pathways, the enzyme's conductivity and stability were improved, leading to enhanced electron transfer and enzymatic stability.

CHEMICAL ENGINEERING JOURNAL (2022)

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Article Chemistry, Physical

Double signal ratiometric electrochemical riboflavin sensor based on macroporous carbon/electroactive thionine-contained covalent organic framework

Na Wu et al.

Summary: An electroactive covalent-organic framework nanobelt was developed for double signal ratiometric electrochemical detection of riboflavin, providing a sensitive and accurate method for riboflavin determination.

JOURNAL OF COLLOID AND INTERFACE SCIENCE (2022)

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Article Chemistry, Analytical

Towards Multi-Analyte Detection with Field-Effect Capacitors Modified with Tobacco Mosaic Virus Bioparticles as Enzyme Nanocarriers

Melanie Welden et al.

Summary: Utilizing tobacco mosaic virus particles as nanoscaffolds, a bi-enzyme EISCAP biosensor was developed for sequential detection of penicillin and urea. The biosensor exhibited high sensitivity and precision, and could also mimic an enzyme logic gate.

BIOSENSORS-BASEL (2022)

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Review Chemistry, Analytical

Hyaluronic Acid Allows Enzyme Immobilization for Applications in Biomedicine

Jackie Arnold et al.

Summary: This review highlights the current applications of enzymes in various industries such as biofuel production, plastics, chemical waste management, detergent, textile, and food production. It also discusses the challenges of transferring enzymes from their natural environment to synthetic practice and the benefits of enzyme immobilization in improving their usage. The article focuses on the use of hyaluronic acid as a platform for enzyme immobilization in the biomedical field and its potential in drug delivery, tissue regeneration, and biological sensors.

BIOSENSORS-BASEL (2022)

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Article Nanoscience & Nanotechnology

Acetylcholinesterase Immobilized on Magnetic Mesoporous Silica Nanoparticles Coupled with Fluorescence Analysis for Rapid Detection of Carbamate Pesticides

Yongan Zhu et al.

Summary: In this study, a magnetic mesoporous material was successfully synthesized and acetylcholinesterase (AChE) was immobilized on it. The magnetic properties of the immobilized AChE improved the detection sensitivity and reliability for pesticide residues.

ACS APPLIED NANO MATERIALS (2022)

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Article Biophysics

Wearable sensors made with solution-blow spinning poly(lactic acid) for non-enzymatic pesticide detection in agriculture and food safety

Rafaella T. Paschoalin et al.

Summary: This paper presents a wearable non-enzymatic electrochemical sensor for the detection of pesticides on the surface of agricultural and food samples. The sensor is low-cost and non-destructive, making it a promising tool for precision agriculture.

BIOSENSORS & BIOELECTRONICS (2022)

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Article Electrochemistry

Novel Electrochemical Acetylcholinesterase Biosensor Based on Core-Shell Covalent Organic Framework@Multi-Walled Carbon Nanotubes (COF@MWCNTs) Composite for Detection of Malathion

Xue Wang et al.

Summary: In this study, a novel electrochemical biosensor based on covalent organic framework@multi-walled carbon nanotubes composite was developed for the detection of organophosphorus pesticides. The biosensor exhibited good sensitivity, anti-interference ability, and stability, and showed satisfactory recovery in water and spinach samples.

INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE (2022)

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Article Chemistry, Analytical

Acetylcholinesterase Biosensor Based on Functionalized Renewable Carbon Platform for Detection of Carbaryl in Food

Erik W. Nunes et al.

Summary: This study describes the functionalization of renewable carbon material through an acid treatment, and the construction of an electrochemical biosensor for the detection of carbaryl pesticide in apple samples. The biosensor showed a low detection limit and high recovery rate, making it a promising renewable tool for food safety.

BIOSENSORS-BASEL (2022)

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Article Engineering, Environmental

Substrate preference of carbamate hydrolase CehA reveals its environmental behavior

Wankui Jiang et al.

Summary: The study found that CehA genes from different hosts are highly conserved but exhibit significant substrate preferences. Point mutations can affect the substrate specificity of CehA genes. Considering the presence of transposable elements in the flanking regions of cehA, it is speculated that hosts may have acquired the ability to hydrolyze carbamate pesticides and their substrate preferences through horizontal gene transfer and genetic copying errors.

JOURNAL OF HAZARDOUS MATERIALS (2021)

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Article Engineering, Environmental

Selective and sensitive multiplexed detection of pesticides in food samples using wearable, flexible glove-embedded non-enzymatic sensors

Paulo A. Raymundo-Pereira et al.

Summary: This study presents a low-cost, portable method for selective and sensitive detection of four classes of pesticides using glove-embedded sensors. The sensors showed high performance in discriminating pesticides in real samples, demonstrating their potential for on-site analysis of chemical threats.

CHEMICAL ENGINEERING JOURNAL (2021)

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Article Chemistry, Analytical

Ratiometric G-Quadruplex Assay for Robust Lead Detection in Food Samples

Yumei Liu et al.

Summary: The ratiometric aptamer probes proposed here can robustly monitor Pb2+ in food samples by leveraging the transition of G-quadruplex structural response to Pb2+, monitored by NMM, and utilizing specific dye and terminal-labeled fluorophore for ratiometric signal outputs towards Pb2+, demonstrating feasibility for on-site Pb2+ testing associated with food safety.

BIOSENSORS-BASEL (2021)

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Article Chemistry, Analytical

Smartphone-Based Dual-Channel Immunochromatographic Test Strip with Polymer Quantum Dot Labels for Simultaneous Detection of Cypermethrin and 3-Phenoxybenzoic Acid

Yuting Zhao et al.

Summary: Gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-MS (LC-MS) are commonly used methods to detect pesticides, but not suitable for on-site biomonitoring. A smartphone-based dual-channel immunochromatographic test strip (ICTS) was developed for simultaneous detection of cypermethrin and its metabolite, promising for accurate point-of-care biomonitoring of pesticide exposure. Polymer carbon dots (PCDs) were used as signal amplifier, showing excellent linear responses for detecting cypermethrin and 3-phenoxybenzoic acid. This innovative device provides a comprehensive analysis of pesticide exposure in a convenient and efficient manner.

ANALYTICAL CHEMISTRY (2021)

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Article Chemistry, Analytical

Duplex-Specific Nuclease-Triggered Fluorescence Immunoassay Based on Dual-Functionalized AuNP for Acetochlor, Metolachlor, and Propisochlor

Yonghong Zha et al.

Summary: This study introduces a double-functionalized gold nanoparticle probe for immunoassay analysis of pesticide residues with high sensitivity and low cost.

ANALYTICAL CHEMISTRY (2021)

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Article Biophysics

A novel biosensor based on multienzyme microcapsules constructed from covalent-organic framework

Huihui Liang et al.

Summary: This study utilized multienzyme microcapsules (enzymes@COF) containing glucose oxidase, horseradish peroxidase and acetylcholinesterase to construct an electrochemical biosensor with successful results. The 600 nm-sized cavity allowed for free conformation expansion of enzymes, while the COF shell provided good chemical stability and protection against external harsh environments. Additionally, the specific catalytic substrates of the enzymes could be infiltrated into the microcapsule through the pores of the COF shell.

BIOSENSORS & BIOELECTRONICS (2021)

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Article Biophysics

Background-free sensing platform for on-site detection of carbamate pesticide through upconversion nanoparticles-based hydrogel suit

Dandan Su et al.

Summary: The study presented a novel portable detection kit for carbaryl pesticide, utilizing UCNPS/PDA nanoprobe and a smartphone detector to accurately quantify carbaryl in complex tea samples. The kit demonstrated high sensitivity, portability, and strong anti-interference capability, making it a promising tool for on-site monitoring of carbaryl in real time.

BIOSENSORS & BIOELECTRONICS (2021)

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Review Chemistry, Inorganic & Nuclear

Colorimetric based on-site sensing strategies for the rapid detection of pesticides in agricultural foods: New horizons, perspectives, and challenges

Reddicherla Umapathi et al.

Summary: Agricultural food crop yields have increased significantly with the use of chemical pesticides, but their excessive use has caused contamination. On-site monitoring of pesticide residues is crucial for global food and environmental safety.

COORDINATION CHEMISTRY REVIEWS (2021)

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Article Environmental Sciences

Residential exposure to carbamate, organophosphate, and pyrethroid insecticides in house dust and risk of childhood acute lymphoblastic leukemia

Jessica M. Madrigal et al.

Summary: A study evaluated the association between household carpet dust measurements of 20 insecticides and childhood acute lymphoblastic leukemia risk. The most frequently detected insecticide active ingredients were permethrin, chlorpyrifos, diazinon, and carbaryl. However, no significant associations with leukemia risk were found among these insecticides.

ENVIRONMENTAL RESEARCH (2021)

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Article Chemistry, Applied

MXene@Ag-based ratiometric electrochemical sensing strategy for effective detection of carbendazim in vegetable samples

Wei Zhong et al.

Summary: In this study, a novel ratiometric electrochemical sensor for carbendazim detection was developed using a composite of MXene@Ag nanoclusters and amino-functionalized multi-walled carbon nanotubes. The sensor exhibited a favorable linear relationship and low detection limit, with high selectivity, good reproducibility, stability, and applicability in vegetable samples.

FOOD CHEMISTRY (2021)

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Article Chemistry, Analytical

Two-Dimensional MnO2 Nanozyme-Mediated Homogeneous Electrochemical Detection of Organophosphate Pesticides without the Interference of H2O2 and Color

Jiahui Wu et al.

Summary: In this study, manganese dioxide nanosheets were used as catalysts to develop a novel electrochemical sensor for organophosphate pesticides detection, eliminating interference from H2O2 and color. The sensor showed improved performance and lower detection limits, providing a new pathway for nanozyme-based sensors and finding more applications in food samples for OP residue determination.

ANALYTICAL CHEMISTRY (2021)

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Article Biophysics

Application of electrochemical methods for the detection of abiotic stress biomarkers in plants

Zhilei Li et al.

Summary: Electrochemical methods have unique advantages in assessing plant hormones and detecting abiotic stress biomarkers, with detection limits that can reach very low levels. However, more efforts are needed to develop portable instruments for detecting endogenous hormones or plant physiological states, as well as research on plant-wearable sensors.

BIOSENSORS & BIOELECTRONICS (2021)

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Article Engineering, Environmental

Conducting microporous organic polymer with -OH functional groups: Special structure and multi-functional integrated property for organophosphorus biosensor

Sheying Dong et al.

Summary: A unique phloroglucinol-based MOP material with abundant -OH functional groups was synthesized and applied for constructing an organophosphate pesticides biosensor. The interaction among OH-POF, AChE, and NF benefited the immobilization of enzymes on the electrode surface, leading to improved catalysis ability for the detection of paraoxon and methyl parathion with wide linear range and low limit of detection. This study highlights the potential value of OH-POF material for electrochemical biosensors and provides a fresh research idea for further exploring MOPs in environmental analysis.

CHEMICAL ENGINEERING JOURNAL (2021)

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Review Chemistry, Analytical

Rapid field trace detection of pesticide residue in food based on surface-enhanced Raman spectroscopy

De Zhang et al.

Summary: This review provides a comprehensive report on the detection of pesticides using SERS technology, discussing the characteristics of different pesticides and the impact of substrates on detection. It highlights the progress in real sample analysis and Raman data processing methods with intelligent algorithms. Future research needs to address the challenges of rapid detection of pesticide residue in complex food matrices using SERS.

MICROCHIMICA ACTA (2021)

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Article Chemistry, Analytical

A novel acetylcholinesterase biosensor with dual-recognized strategy based on molecularly imprinted polymer

Jun-feng Qi et al.

Summary: A novel dual-recognized strategy using MIP and AChE was developed to detect pesticides, providing increased selectivity and accuracy in identifying enzyme inhibitors in complex samples using enzyme-based biosensors.

SENSORS AND ACTUATORS B-CHEMICAL (2021)

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Article Chemistry, Analytical

Sponge-like CuInS2 microspheres on reduced graphene oxide as an electrocatalyst to construct an immobilized acetylcholinesterase electrochemical biosensor for chlorpyrifos detection in vegetables

Teerapat Itsoponpan et al.

Summary: A practical electrochemical biosensor using copper indium sulfide and reduced graphene oxide microspheres was reported for detecting chlorpyrifos in vegetables. The sensor showed high sensitivity, low detection limit, wide linear range, and satisfactory accuracy in detecting chlorpyrifos in vegetable samples.

SENSORS AND ACTUATORS B-CHEMICAL (2021)

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Article Food Science & Technology

Application of surface-enhanced Raman spectroscopy using silver and gold nanoparticles for the detection of pesticides in fruit and fruit juice

Su-Yan Wang et al.

Summary: This study comprehensively covers all reported methods for the detection of pesticides in fruit and fruit juice using SERS enhanced with gold and silver nanoparticles, comparing detection parameters obtained under different experimental conditions. The achievements and limitations of this technique for pesticide detection in fruit and fruit juice are discussed.

TRENDS IN FOOD SCIENCE & TECHNOLOGY (2021)

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Article Chemistry, Applied

Two-dimensional self-assembled Au -Ag core-shell nanorods nanoarray for sensitive detection of thiram in apple using surface-enhanced Raman spectroscopy

Hongbin Pu et al.

Summary: The study developed a two-dimensional Au-Ag core-shell nanorods nanoarray substrate that showed excellent performance for SERS detection of contaminants in foods, with high sensitivity and stability, achieving good detection results for thiram and other contaminants.

FOOD CHEMISTRY (2021)

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Article Chemistry, Applied

Dispersive solid-phase extraction using microporous metal-organic framework UiO-66: Improving the matrix compounds removal for assaying pesticide residues in organic and conventional vegetables

Xuejin Mao et al.

Summary: An efficient dispersive solid-phase extraction method using UiO-66 as sorbent has been developed for tracing pesticide residues in vegetables. The method demonstrates good extraction efficiency and reusability, with optimized conditions achieving excellent detection performance.

FOOD CHEMISTRY (2021)

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Article Chemistry, Physical

2D Ti3C2Tx flakes prepared by in-situ HF etchant for simultaneous screening of carbamate pesticides

Ankita Sinha et al.

Summary: The present study involves the preparation of colloidal single/few layer Ti3C2Tx MXene flakes using minimally intensive layer delamination (MILD) method for rapid electroanalytical screening of carbamate pesticides. The delaminated Ti3C2Tx flakes were used as a working electrode for simultaneous and selective detection of carbamate pesticides methiocarb and diethofencarb, exhibiting good stability and recoveries for environmental applications.

JOURNAL OF COLLOID AND INTERFACE SCIENCE (2021)

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Article Chemistry, Analytical

A New Covalent Organic Framework of Dicyandiamide-Benzaldehyde Nanocatalytic Amplification SERS/RRS Aptamer Assay for Ultratrace Oxytetracycline with the Nanogold Indicator Reaction of Polyethylene Glycol 600

Aihui Liang et al.

Summary: In this study, a new type of high catalytic COF material DdBd was successfully prepared, which was used to catalyze AuNP formation and develop a new dual-mode scattering SERS/RRS method for quantitative analysis of ultratrace OTC.

BIOSENSORS-BASEL (2021)

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Article Chemistry, Analytical

Nanozyme-Participated Biosensing of Pesticides and Cholinesterases: A Critical Review

Hengjia Zhu et al.

Summary: Pesticides play a vital role in protecting crops, but their difficult-to-degrade residues pose threats to human health and the environment. Nanomaterials with enzyme-like properties, known as nanozymes, offer great potential in detecting pesticide residues and biomarkers efficiently.

BIOSENSORS-BASEL (2021)

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Article Chemistry, Analytical

Electrochemical Biosensor for Markers of Neurological Esterase Inhibition

Neda Rafat et al.

Summary: A novel experimental and modeling framework was applied to an inhibition-based bi-enzyme electrochemical biosensor to detect AChE inhibitors. The biosensor was fabricated with a redox-recycle amplification mechanism and a mathematical model was used to simulate diffusion and reaction steps, showing its utility in characterizing and optimizing biosensor performance. The model was also used to calculate sensitivity, amplification factor, Damkohler numbers, and current-control coefficients, and to discuss design and operating conditions for achieving specific performance criteria.

BIOSENSORS-BASEL (2021)

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Article Chemistry, Analytical

Bifunctional Moderator-Powered Ratiometric Electrochemiluminescence Enzymatic Biosensors for Detecting Organophosphorus Pesticides Based on Dual-Signal Combined Nanoprobes

Ying He et al.

Summary: A novel dual-signal combined nanoprobe with two different types of nanoparticles as ECL probes and hydrogen peroxide as a bifunctional moderator was designed for enzyme sensing analysis. In the presence of organophosphorus compounds, enzyme activity was inhibited, leading to changes in ECL signals. This integrated system provides a new pathway for ratiometric ECL systems involving enzyme catalytic amplification.

ANALYTICAL CHEMISTRY (2021)

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Article Engineering, Environmental

Rational hapten design to produce high-quality antibodies against carbamate pesticides and development of immunochromatographic assays for simultaneous pesticide screening

Zi-Jian Chen et al.

Summary: Carbamate pesticides are widely used in agricultural production and pest control. This study proposed a general hapten strategy based on carbamate moiety recognition to obtain specific monoclonal antibodies for carbofuran, isoprocarb, and carbaryl. An immunochromatographic assay was developed using these antibodies to simultaneously detect the three pesticides with good potential for rapid and simultaneous detection of multiple pesticide residues.

JOURNAL OF HAZARDOUS MATERIALS (2021)

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Article Engineering, Environmental

Aggregation-induced emission luminogen@manganese dioxide core-shell nanomaterial-based paper analytical device for equipment-free and visual detection of organophosphorus pesticide

Jianling Chen et al.

Summary: A high-performance fluorescence paper analytical device (PAD) was developed for equipment-free and visual sensing of organophosphorus pesticide residues, providing a new avenue to bring OPs analysis out of the lab and into the world.

JOURNAL OF HAZARDOUS MATERIALS (2021)

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Article Chemistry, Analytical

Ratiometric electrochemiluminescence biosensor based on Ir nanorods and CdS quantum dots for the detection of organophosphorus pesticides

Guomin Yang et al.

Summary: This study prepared water-soluble Ir nanorods and developed a highly sensitive dual-signal nanoprobe for detecting organophosphorus pesticides, utilizing the opposite changes in two ECL signals for target detection.

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Article Chemistry, Analytical

Efficient acetylcholinesterase immobilization for improved electrochemical performance in polypyrrole nanocomposite-based biosensors for carbaryl pesticide

Lara F. Loguercio et al.

Summary: Efficient and portable sensors are needed for monitoring pesticide contaminants in various environments. This study focused on a biocomposite film, which optimized surface conditions for enzyme immobilization and showed improved catalytic performance. The biosensor demonstrated linear responses for carbaryl detection with good repeatability and reproducibility, and improved stability at lower temperatures.

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Article Chemistry, Multidisciplinary

2D and 3D Porphyrinic Covalent Organic Frameworks: The Influence of Dimensionality on Functionality

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ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2020)

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The effectiveness of chemical solutions on the removal of carbaryl residues from cucumber and chili presoaked in carbaryl using the HPLC technique

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Biosensor Based on Covalent Organic Framework Immobilized Acetylcholinesterase for Ratiometric Detection of Carbaryl

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