Journal
BIOSENSORS-BASEL
Volume 12, Issue 8, Pages -Publisher
MDPI
DOI: 10.3390/bios12080632
Keywords
biosensor; laccase; hydroxyl fullerenes; multi-walled carbon nanotubes; rutin determination
Funding
- National Natural Science Foundation of China (NSFC) [32161143021]
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In this study, a biosensor based on multi-walled carbon nanotubes and laccase nanocomposite-modified electrodes was developed for the sensitive and selective detection of rutin. The biosensor showed a wide linear range and low detection limits, and demonstrated high accuracy in detecting rutin tablets and serum samples. This biosensor has potential practical applications in rutin drug quality testing and clinical blood drug concentration monitoring.
Rutin is a flavonoid glycoside compound, which is mainly transported via the blood circulation system in the human body. The monitoring of the blood concentration of rutin is of great significance in many fields such as pharmacology and pharmacokinetics. In this work, a biosensor based on multi-walled carbon nanotubes (MWCNTs), cetyltrimethylammonium bromide (CTAB), hydroxyl fullerenes (HFs), and laccase (Lac) nanocomposite-modified glassy carbon electrodes was constructed. The modified materials were characterized with a transmission electron microscope (TEM), cyclic voltammograms (CV), and electrochemical impedance spectroscopy (EIS). CTAB is used to disperse MWCNTs and improve hydrophilicity and biocompatibility of MWCNTs, while the use of Lac can enhance the oxidation of catechol structure in rutin, thus significantly improving the sensitivity and selectivity of the modified electrode. Linear sweep voltammetry (LSV) studies showed that the determination linear ranges of rutin were 0.1 mu mol L-1 to 2 mu mol L-1 and 2 mu mol L-1 to 11 mu mol L-1, with the determination limits of 30 nmol L-1 and 95.5 nmol L-1, respectively. The proposed biosensor can be used to detect rutin tablets and serum samples with high recovery, which indicates a good accuracy of this method, and the results are consistent with those measured by the traditional ultra-high performance liquid chromatography (UHPLC) method. Hence, this biosensor has potential practical application value in rutin drug quality testing and clinical blood drug concentration monitoring.
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