4.6 Article

Characterization of Structure and Antioxidant Activity of Polysaccharides From Sesame Seed Hull

Journal

FRONTIERS IN NUTRITION
Volume 9, Issue -, Pages -

Publisher

FRONTIERS MEDIA SA
DOI: 10.3389/fnut.2022.928972

Keywords

sesame seed; hull; polysaccharides; chemical structure; antioxidant activity

Funding

  1. Natural Science Foundation of Excellent Youth for Henan [222300420038]
  2. Key Project of Science and Technology of Henan Province [201300110600]
  3. Innovation and Entrepreneurship Training Program for Higher Education Students in Henan Province [202110463042, 202110463043, 202110463040]

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In this study, sesame hull polysaccharides (SHP) were successfully extracted using ultrasound-assisted alkali extraction methods. Three purified polysaccharide fractions were obtained, with SHP-2 being the dominant fraction. It was found that SHP-2 had a high yield and contained various types of polysaccharides. This study provides useful basic data for the development of applications for sesame seed hull polysaccharides in the food and pharmaceutical industries.
Sesame seed hull is the major by-product of sesame seed processing and is rich in polysaccharides. In this work, sesame hull polysaccharides (SHP) were extracted by ultrasound-assisted alkali extraction methods with a yield of 6.49%. Three purified polysaccharide fractions were obtained after decolorization, deproteinization, and column chromatography. Then, their main composition and antioxidant activity were investigated. The dominant fraction was SHP-2 with a yield of 3.78%. It was composed of galacturonic acid (51.3%), glucuronic acid (13.8%), rhamnose (8.9%), glucose (8.4%), and others. The linkage types of SHP-2 have the alpha-D-GalpA-(1,4)-linked, alpha-D-GlcpA-(1,2)-linked, beta-T-D-Rhap-linked, beta-D-Glcp-(1,6)-linked, beta-T-D-Galp-linked, alpha-L-Xylp-(1,4)-linked, alpha-L-Araf-(1,3,5)-linked, and beta-D-Manp-(1,4)-linked. This study might provide some useful basic data for developing applications for sesame seed hull polysaccharides in the food and pharmaceutical industries.

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