Response of Cyanic and Acyanic Lettuce Cultivars to an Increased Proportion of Blue Light

Simple Summary Indoor crop cultivation systems such as vertical farms or plant factories necessitate artificial lighting. The composition of light quality (i.e., spectral composition) within these systems plays a key role in crop growth and development. Conflicting results on the effects of the light spectrum reported for different plant species and cultivars confirm the specificity of light requirements and the dependency on interacting factors. In this paper, we have therefore investigated how a certain light quality (light with a high share of blue) affects photosynthetic and morphological parameters in two contrasting lettuce cultivars (red and green leaves) with a similar leaf shape and phenotype. The results obtained suggest the occurrence of distinctive morpho-physiological adaptive strategies in green and red pigmented lettuce cultivars to adapt to the higher proportion of blue light environment. Indoor crop cultivation systems such as vertical farms or plant factories necessitate artificial lighting. Light spectral quality can affect plant growth and metabolism and, consequently, the amount of biomass produced and the value of the produce. Conflicting results on the effects of the light spectrum in different plant species and cultivars make it critical to implement a singular lighting solution. In this study we investigated the response of cyanic and acyanic lettuce cultivars to an increased proportion of blue light. For that, we selected a green and a red leaf lettuce cultivar (i.e., 'Aquino', CVg, and 'Barlach', CVr, respectively). The response of both cultivars to long-term blue-enriched light application compared to a white spectrum was analyzed. Plants were grown for 30 days in a growth chamber with optimal environmental conditions (temperature: 20 degrees C, relative humidity: 60%, ambient CO2, photon flux density (PFD) of 260 mu mol m(-2) s(-1) over an 18 h photoperiod). At 15 days after sowing (DAS), white spectrum LEDs (WW) were compared to blue-enriched light (WB; lambda(Peak) = 423 nm) maintaining the same PFD of 260 mu mol m(-2) s(-1). At 30 DAS, both lettuce cultivars adapted to the blue light variant, though the adaptive response was specific to the variety. The rosette weight, light use efficiency, and maximum operating efficiency of PSII photochemistry in the light, F-v/F-m', were comparable between the two light treatments. A significant light quality effect was detected on stomatal density and conductance (20% and 17% increase under WB, respectively, in CVg) and on the modified anthocyanin reflectance index (mARI) (40% increase under WB, in CVr). Net photosynthesis response was generally stronger in CVg compared to CVr; e.g., net photosynthetic rate, P-n, at 1000 mu mol m(-2) s(-1) PPFD increased from WW to WB by 23% in CVg, compared to 18% in CVr. The results obtained suggest the occurrence of distinct physiological adaptive strategies in green and red pigmented lettuce cultivars to adapt to the higher proportion of blue light environment.

Automated summary

Indoor crop cultivation systems require artificial lighting, and the quality of light can affect plant growth and metabolism, as well as the biomass produced and the value of the produce. This study investigated how a certain light quality, with a high proportion of blue light, affected photosynthetic and morphological parameters in two lettuce cultivars with different leaf colors. The results suggest that there are distinct physiological adaptive strategies in green and red pigmented lettuce cultivars to adapt to a higher proportion of blue light environment.