Multimodal imaging of the dynamic brain tumor microenvironment during glioblastoma progression and in response to treatment

Tumors evolve in a dynamic communication with their native tissue environment and recruited immune cells. The diverse components of the tumor microenvironment (TME) can critically regulate tumor progression and therapeutic response. In turn, anticancer treatments may alter the composition and functions of the TME. To investigate this continuous dialog in the context of primary brain cancers, we developed a multimodal longitudinal imaging strategy. We combined macroscopical magnetic resonance imaging with subcellular resolution two-photon intravital microscopy, and leveraged the power of single-cell analysis tools to gain insights into the ongoing interactions between different compo-nents of the TME and cancer cells. Our experiments revealed that the migratory behavior of tumor-associated macrophages is different in genetically distinct glioblastomas, and in response to macrophage-targeted therapy. These results underscore the importance of studying cancer longitudinally in an in vivo setting, to reveal complex and dynamic alterations in the TME during disease progression and therapeutic intervention.

Automated summary

Tumors rely on continuous communication with their surrounding tissue and immune cells. By developing a multimodal imaging strategy, researchers discovered that the migratory behavior of tumor-associated macrophages varies in different genetically distinct glioblastomas and in response to targeted therapy. These findings highlight the importance of studying tumors longitudinally in vivo to understand the complex and dynamic changes in the tumor microenvironment during disease progression and treatment intervention.