Anhydrobiotic chironomid larval motion-based multi-sensing microdevice for the exploration of survivable locations

African chironomid (Polypedilum vanderplanki) larvae can suspend their metabolism by undergoing severe desiccation and then resume this activity by simple rehydration. We present a microdevice using interdigital comb electrodes to detect the larval motion using the natural surface charge of the living larvae in water The larvae were most active 2 h after soaking them in water at 30 degrees C; they exhibited motions with 2 Hz frequency. This was comparable to the signal obtained from the microdevice via fast Fourier transform (FFT) processing. The amplitude of the voltage and current were 0.11 mV and 730 nA, respectively. They would be enough to be detected by a low power consumption microcomputer. Temperature and pH sensing were demonstrated by detecting the vital motions of the revived larvae under different conditions. This multi-functional biosensor will be a useful microdevice to search for survivable locations under extreme environmental conditions like those on other planets.

Automated summary

African chironomid larvae can suspend their metabolism through desiccation and resume activity through rehydration. A microdevice using interdigital comb electrodes was developed to detect the larval motion by leveraging their natural surface charge. This biosensor demonstrated temperature and pH sensing capabilities, making it valuable for searching survivable locations in extreme environments.