Journal
BIOMEDICINES
Volume 10, Issue 8, Pages -Publisher
MDPI
DOI: 10.3390/biomedicines10081810
Keywords
umbilical cord-derived; extracellular vesicles; mesenchymal stem cells; skin rejuvenation; fibroblast migration; fibroblast proliferation; extracellular matrix
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This study investigates the roles of different EV sub-populations released by TGF-beta-primed UCMSCs and their ability to alter dermal fibroblast functions. The results suggest that TGF-beta-stimulated UCMSCs derived EVs have the potential to promote fibroblast migration and enhance ECM protein production, which may have a protective effect against skin aging.
Umbilical cord-derived mesenchymal stem cells (UCMSCs) have been illustrated for their roles in immunological modulation and tissue regeneration through the secretome. Additionally, culture conditions can trigger the secretion of extracellular vesicles (EVs) into extracellular environments with significant bioactivities. This study aims to investigate the roles of three EV sub-populations released by UCMSCs primed with transforming growth factor beta (TGF beta) and their capacity to alter dermal fibroblast functions for skin aging. Results show that three EV sub-populations, including apoptotic bodies (ABs), microvesicles (MVs), and exosomes (EXs), were separated from conditioned media. These three EVs carried growth factors, such as FGF-2, HGF, and VEGF-A, and did not express noticeable effects on fibroblast proliferation and migration. Only EX from TGF beta-stimulated UCMSCs exhibited a better capacity to promote fibroblasts migrating to close scratched wounds than EX from UCMSCs cultured in the normal condition from 24 h to 52 h. Additionally, mRNA levels of ECM genes (COL I, COL III, Elastin, HAS II, and HAS III) were detected with lower levels in fibroblasts treated with EVs from normal UCMSCs or TGF beta-stimulated UCMSCs compared to EV-depleted condition. On the contrary, the protein levels of total collagen and elastin released by fibroblasts were greater in the cell groups treated with EVs compared to EV-depleted conditions; particularly elastin associated with TGF beta-stimulated UCMSCs. These data indicate the potential roles of EVs from UCMSCs in protecting skin from aging by promoting ECM protein production.
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