☆ 4.6 Article

Directed evolution of an efficient and thermostable PET depolymerase

NATURE CATALYSIS (2022)

Related references

Note: Only part of the references are listed.

Article Chemistry, Multidisciplinary

Comparative Performance of PETase as a Function of Reaction Conditions, Substrate Properties, and Product Accumulation

Erika Erickson et al.

Summary: This study compares the performance of two types of PET-hydrolyzing enzymes and finds that reaction temperature has the strongest influence on their performance. Both enzymes achieve higher levels of PET conversion for substrates with moderate crystallinity, and show different inhibition profiles to product accumulation.

CHEMSUSCHEM (2022)

Add to Collection

Review Biotechnology & Applied Microbiology

Enzyme discovery and engineering for sustainable plastic recycling

Baotong Zhu et al.

Summary: The increasing amount of plastic waste is causing an environmental crisis, requiring innovative technologies for recycling. Biocatalytic depolymerization mediated by enzymes is an efficient and sustainable method for plastic treatment, with protein engineering used to optimize enzyme performance.

TRENDS IN BIOTECHNOLOGY (2022)

Add to Collection

Article Engineering, Environmental

The future of UK plastics recycling: One Bin to Rule Them All

Martin Burgess et al.

Summary: The use of plastics in the UK is increasingly demonised, with public campaigns advocating for recyclable cups and the elimination of plastic straws. However, despite efforts from retailers and the UK Plastics Pact, domestic collection rates for discarded plastics remain low. Research suggests that rapid progress in standardisation, infrastructure investment, cross-supply chain business models, and higher value recyclate is needed to significantly improve recycling rates. A harmonised national solution to plastic waste sorting, while maintaining value in discarded plastics, could serve as a best-practice model for other regions.

RESOURCES CONSERVATION AND RECYCLING (2021)

Add to Collection

Article Chemistry, Physical

Computational Redesign of a PETase for Plastic Biodegradation under Ambient Condition by the GRAPE Strategy

Yinglu Cui et al.

Summary: This study successfully improved the robustness of PETase through a computational strategy, resulting in the redesign of a variant DuraPETase with significantly elevated melting temperature and enhanced degradation of PET films, as well as complete biodegradation of microplastics at mild temperatures. The design strategy presents opportunities for handling uncollectable PET waste and converting resulting monomers into high-value molecules.

ACS CATALYSIS (2021)

Add to Collection

Article Multidisciplinary Sciences

An absorbance method for analysis of enzymatic degradation kinetics of poly(ethylene terephthalate) films

En Ze Linda Zhong-Johnson et al.

Summary: Increased interest in PET-degrading enzymes has led to efforts to find mutants with improved catalytic activity and thermostability. A simple and fast method for determining relative enzyme kinetics was presented in this study, showing that mutations in the thermostable variant of PETase improved catalytic rates 5- to 7-fold. The results support the necessity of kinetic measurements to determine relationships between sequence and function for PET hydrolases like IsPETase.

SCIENTIFIC REPORTS (2021)

Add to Collection

Article Biotechnology & Applied Microbiology

Enhancing PET hydrolytic enzyme activity by fusion of the cellulose-binding domain of cellobiohydrolase I from Trichoderma reesei

Longhai Dai et al.

Summary: The fusion of a cellulose-binding domain CBM onto IsPETaseEHA was found to significantly enhance the enzymatic hydrolysis of PET, particularly at higher temperatures. This strategy shows promise in stimulating the biodegradation of PET.

JOURNAL OF BIOTECHNOLOGY (2021)

Add to Collection

Review Chemistry, Physical

Chemical and biological catalysis for plastics recycling and upcycling

Lucas D. Ellis et al.

Summary: Plastic pollution is causing an environmental crisis, driving the development of new recycling and upcycling methods. Both chemical and biological catalysis face challenges and opportunities, requiring innovative design to overcome limitations.

NATURE CATALYSIS (2021)

Add to Collection

Article Chemistry, Physical

Techno-economic, life-cycle, and socioeconomic impact analysis of enzymatic recycling of poly(ethylene terephthalate)

Avantika Singh et al.

Summary: Esterases play a crucial role in enzymatic PET recycling, enabling efficient depolymerization to TPA and ethylene glycol, reducing energy consumption and greenhouse gas emissions, and generating more socio-economic benefits.

JOULE (2021)

Add to Collection

Article Chemistry, Multidisciplinary

Microbial synthesis of vanillin from waste poly(ethylene terephthalate)

Joanna C. Sadler et al.

Summary: This study reports a novel approach to directly upcycle post-consumer PET waste into valuable vanillin using engineered Escherichia coli. After process optimization, a conversion rate of 79% from terephthalic acid to vanillin was achieved, marking a significant improvement in yield. This work demonstrates the first biological upcycling of post-consumer plastic waste into vanillin, showcasing the potential for sustainable solutions in the plastic recycling industry.

GREEN CHEMISTRY (2021)

Add to Collection

Article Chemistry, Physical

General features to enhance enzymatic activity of poly(ethylene terephthalate) hydrolysis

Chun-Chi Chen et al.

Summary: The mutational strategy of replacing His/Phe residues with Ser/Ile has been found to enhance the PET-hydrolytic activity of enzymes, providing an important approach for improving the activity of potential PET-hydrolytic enzymes.

NATURE CATALYSIS (2021)

Add to Collection

Article Multidisciplinary Sciences

An engineered PET depolymerase to break down and recycle plastic bottles

V. Tournier et al.

NATURE (2020)

Add to Collection

Review Multidisciplinary Sciences

Biocatalytic recycling of polyethylene terephthalate plastic

Wolfgang Zimmermann

PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY A-MATHEMATICAL PHYSICAL AND ENGINEERING SCIENCES (2020)

Add to Collection

Article Multidisciplinary Sciences

Characterization and engineering of a two-enzyme system for plastics depolymerization

Brandon C. Knott et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2020)

Add to Collection

Article Biotechnology & Applied Microbiology

Structural bioinformatics-based protein engineering of thermo-stable PETase from Ideonella sakaiensis

Hyeoncheol Francis Son et al.

ENZYME AND MICROBIAL TECHNOLOGY (2020)

Add to Collection

Article Chemistry, Multidisciplinary

Towards closed-loop recycling of multilayer and coloured PET plastic waste by alkaline hydrolysis

Sibel Ugduler et al.

GREEN CHEMISTRY (2020)

Add to Collection

Article Chemistry, Physical

Rational Protein Engineering of Thermo-Stable PETase from Ideonella sakaiensis for Highly Efficient PET Degradation

Hyeoncheol Francis Son et al.

ACS CATALYSIS (2019)

Add to Collection

Article Chemistry, Physical

Combining Reclaimed PET with Bio-based Monomers Enables Plastics Upcycling

Nicholas A. Rorrer et al.

JOULE (2019)

Add to Collection

Article Chemistry, Multidisciplinary

Organocatalysed depolymerisation of PET in a fully sustainable cycle using thermally stable protic ionic salt

Coralie Jehanno et al.

GREEN CHEMISTRY (2018)

Add to Collection

Article Multidisciplinary Sciences

Characterization and engineering of a plastic-degrading aromatic polyesterase

Harry P. Austin et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2018)

Add to Collection

Article Multidisciplinary Sciences

Structural insight into molecular mechanism of poly (ethylene terephthalate) degradation

Seongjoon Joo et al.

NATURE COMMUNICATIONS (2018)

Add to Collection

Review Biochemistry & Molecular Biology

Directed Evolution of Protein Catalysts

Cathleen Zeymer et al.

ANNUAL REVIEW OF BIOCHEMISTRY, VOL 87 (2018)

Add to Collection

Article Engineering, Multidisciplinary

Enhanced Poly(ethylene terephthalate) Hydrolase Activity by Protein Engineering

Yuan Ma et al.

ENGINEERING (2018)

Add to Collection

Article Chemistry, Multidisciplinary

Directed Evolution: Bringing New Chemistry to Life

Frances H. Arnold

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2018)

Add to Collection

Article Multidisciplinary Sciences

Structural insight into catalytic mechanism of PET hydrolase

Xu Han et al.

NATURE COMMUNICATIONS (2017)

Add to Collection

Article Multidisciplinary Sciences

Production, use, and fate of all plastics ever made

Roland Geyer et al.

SCIENCE ADVANCES (2017)

Add to Collection

Editorial Material Biotechnology & Applied Microbiology

Biocatalysis as a green route for recycling the recalcitrant plastic polyethylene terephthalate

Ren Wei et al.

MICROBIAL BIOTECHNOLOGY (2017)

Add to Collection

Review Chemistry, Multidisciplinary

Chemical recycling of waste plastics for new materials production

AliReza Rahimi et al.

NATURE REVIEWS CHEMISTRY (2017)

Add to Collection

Article Biochemistry & Molecular Biology

Automated Structure- and Sequence-Based Design of Proteins for High Bacterial Expression and Stability

Adi Goldenzweig et al.

MOLECULAR CELL (2016)

Add to Collection

Review Multidisciplinary Sciences

The coming of age of de novo protein design

Po-Ssu Huang et al.

NATURE (2016)

Add to Collection

Article Multidisciplinary Sciences

A bacterium that degrades and assimilates poly(ethylene terephthalate)

Shosuke Yoshida et al.

SCIENCE (2016)

Add to Collection

Article Biochemistry & Molecular Biology

A disulfide bridge in the calcium binding site of a polyester hydrolase increases its thermal stability and activity against polyethylene terephthalate

Johannes Then et al.

FEBS OPEN BIO (2016)

Add to Collection

Article Multidisciplinary Sciences

Plastic waste inputs from land into the ocean

Jenna R. Jambeck et al.

SCIENCE (2015)

Add to Collection

Article Biotechnology & Applied Microbiology

A novel Ca2+-activated, thermostabilized polyesterase capable of hydrolyzing polyethylene terephthalate from Saccharomonospora viridis AHK190

Fusako Kawai et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2014)

Add to Collection

Article Biotechnology & Applied Microbiology

Isolation of a Novel Cutinase Homolog with Polyethylene Terephthalate-Degrading Activity from Leaf-Branch Compost by Using a Metagenomic Approach

Sintawee Sulaiman et al.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2012)

Add to Collection

Article Biotechnology & Applied Microbiology

Hydrolysis of Polyethyleneterephthalate by p-Nitrobenzylesterase from Bacillus subtilis

Doris Ribitsch et al.

BIOTECHNOLOGY PROGRESS (2011)

Add to Collection

Article Polymer Science

Enzymatic Surface Hydrolysis of PET: Effect of Structural Diversity on Kinetic Properties of Cutinases from Thermobifida

Enrique Herrero Acero et al.

MACROMOLECULES (2011)

Add to Collection

Article Biochemical Research Methods

BglBricks: A flexible standard for biological part assembly

J Christopher Anderson et al.

Journal of Biological Engineering (2010)

Add to Collection

Article Polymer Science

Cutinase-Catalyzed Hydrolysis of Poly(ethylene terephthalate)

Asa M. Ronkvist et al.

MACROMOLECULES (2009)

Add to Collection

Article Biochemical Research Methods

Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes

Manfred T. Reetz et al.

NATURE PROTOCOLS (2007)

Add to Collection

Article Engineering, Chemical

Poly(ethylene terephthalate) recycling and recovery of pure terephthalic acid by alkaline hydrolysis

GP Karayannidis et al.

ADVANCES IN POLYMER TECHNOLOGY (2002)

Add to Collection

© Peeref 2019-2024. All rights reserved.