Journal
FRONTIERS IN PUBLIC HEALTH
Volume 10, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fpubh.2022.975720
Keywords
diarrhea; rotavirus; detection; AlphaLISA; homogeneous assay
Categories
Funding
- Chinese State Key Project Specialized for Infectious Disease
- [2018ZX10711001-003-001]
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In this study, a rapid, accurate, and sensitive AlphaLISA method was developed and evaluated for the detection of rotavirus infection. The results showed good consistency with the gold standard RT-qPCR method. AlphaLISA can be used for the rapid detection of rotavirus infection in clinical practice.
Rotavirus is the main pathogen causing acute viral gastroenteritis. Accurate and rapid diagnosis of rotavirus infection is important to determine appropriate treatment, prevention of unnecessary antibiotics use and control of infection spread. In this study, we established a rapid, accurate, and sensitive amplified luminescent proximity homogeneous assay linked immunosorbent assay (AlphaLISA) for detecting rotavirus and evaluated its efficacy in human stool samples. Our results demonstrated that the sensitivity of AlphaLISA (5(-8)) significantly exceeded that of the immunochromatographic assay (ICA, 5(-4)) for rotavirus antigen detection. The intra-assay and inter-assay coefficients of variation were 2.99-3.85% and 5.27-6.51%, respectively. Furthermore, AlphaLISA was specific for rotavirus and did not cross-react with other common diarrhea viruses. AlphaLISA and real-time reverse transcription polymerase chain reaction (RT-qPCR, which is considered a gold standard for detecting diarrhea viruses) tests showed consistent results on 235 stool samples, with an overall consistency rate of 97.87% and a kappa value of 0.894 (P < 0.001). The overall consistency rate of ICA compared with RT-qPCR was 95.74%. AlphaLISA showed better consistency with RT-qPCR than the routinely used ICA for rotavirus detection in stool samples. The AlphaLISA method can be used in clinical practice for the rapid, accurate, and sensitive detection of rotavirus infection.
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