Corydalis bungeana Turcz. attenuates LPS-induced inflammatory responses via the suppression of NF-κB signaling pathway in vitro and in vivo

Ethnopharmacological relevance: Corydalis bungeana Turcz. (C. bungeana) is one of traditionally used medicines in China and possesses various biological effects, such as anti-inflammatory, antibacterial activity and inhibition of the immune function of the host. Aim of the study: we studied the anti-inflammatory effect and molecular mechanism involved of C. bungeana both in vitro and in vivo model system in which the inflammatory response was induced by LPS treatment. Materials and methods: Anti-inflammatory activity of C. bungeana was investigated by LPS-induced RAW 264.7 macrophages and BALB/c mice. The production and expression of pro-inflammatory cytokines were evaluated by Griess reagent, ELISA kits and RT-OCR, respectively. Phosphorylation status of I kappa B alpha and p65 was illustrated by western blot assay. Results: C. bungeana reduced the secretion of NO, TNF-alpha, IL-6 and IL-1 beta through inhibiting the protein expression of iNOS, TNF-alpha, IL-6 and IL-1 beta in vitro and in vivo. Western blot analysis suggested that C. bungeana supressed NF-kappa B activation via regulating the phosphorylation of I kappa B alpha and p65. Immunohistochemical assay also demostrated the histological inflammatory change in liver tissue. Conclusions: The results indicate that C. bungeana supresses the activation of NF-kappa B signaling pathway through inhibiting phosphorylation of I kappa B alpha and p65, which results in good anti-inflammatory effect. In addition, C. bungeana attenuates inflammatory reaction by supressing the expression of various inflammatory cytokines both in vivo and in vitro. (C) 2016 Elsevier Ireland Ltd. All rights reserved.