Investigation of the Fluorescence Turn-off Mechanism, Genome, Molecular Docking In Silico and In Vitro Studies of 2-Acetyl-3H-benzo[f]chromen-3-one

The present study harnesses fluorescence quenching between a nonfluorescent aniline and fluorophore 2-acetyl-3H- benzo[f]chromen-3-one [2AHBC] in binary solvent mixtures of acetonitrile and 1,4-dioxane at room temperature and explores the fluorophore as an antimicrobial material. Our findings throw light on the key performance of organic molecules in the medicinal and pharmaceutical fields, which are considered as the most leading drives in therapeutic applications. In view of that, fluorescence quenching data have been interpreted by various quenching models. This demonstrates that the sphere of action holds very well in the present work and also confirms the presence of static quenching reactions. Additionally, the fluorophore was first investigated for druglike activity with the help of in silico tools, and then it was investigated for antimicrobial activity through bioinformatics tools, which has shown promising insights.

Automated summary

This study investigates the fluorescence quenching behavior of a nonfluorescent aniline and a fluorophore in binary solvent mixtures and explores the antimicrobial potential of the fluorophore. The findings shed light on the importance of organic molecules in medicine and pharmaceuticals, particularly in therapeutic applications. The study demonstrates the effectiveness of the quenching models used and confirms the presence of static quenching reactions. Additionally, in silico and bioinformatics tools were employed to assess the druglike and antimicrobial activities of the fluorophore, yielding promising insights.