4.6 Article

Simple and Rapid Endotoxin Recognition Using a Dipicolylamine- Modified Fluorescent Probe with Picomolar-Order Sensitivity

Journal

ACS OMEGA
Volume 7, Issue 29, Pages 25891-25897

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/acsomega.2c02935

Keywords

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Funding

  1. Japan Society for the Promotion of Science (JSPS) [18K05180, 20H02772]

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In this study, a simple and rapid method for detecting endotoxin was developed using a dpa-modified pyrenyl probe. The probe exhibited fluorescence in the presence of LPS and had high sensitivity and selectivity for LPS. This probe has the potential to be used as a chemical sensor for the detection of endotoxin in medical and pharmaceutical applications.
Endotoxin is a lipopolysaccharide (LPS) that is found in the outer membrane of the cell wall of Gram-negative bacteria. Due to its high toxicity, the allowable endotoxin limit for water for injection is set at a very low value. Conventional methods for endotoxin detection are time-consuming and expensive and have low reproducibility. A previous study has shown that dipicolylamine (dpa)-modified pyrene-based probes exhibit fluorescence enhancement in response to LPS; however, the application of such probes to the sensing of LPS is not discussed. Against this backdrop, we have developed a simple and rapid endotoxin detection method using a dpa-modified pyrenyl probe having a zinc(II) center (Zn-dpa-C4Py). When LPS was added into Zndpa-C4Py solution, excimer emission of the pyrene moiety emerged at 470 nm. This probe can detect picomolar concentrations of LPS (limit of detection = 41 pM). The high sensitivity of the probe is ascribed to the electrostatic and hydrophobic interactions between the probe and LPS, which result in the dimer formation of the pyrene moieties. We also found that Zn-dpa-C4Py has the highest selectivity for LPS compared with other phosphate derivatives, which is probably caused by the co-aggregation of the probe with LPS. We propose that Zn-dpa-C4Py is a promising chemical sensor for the detection of endotoxin in medical and pharmaceutical applications.

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