Investigating the Effect of the Interaction of Maize Inducer and Donor Backgrounds on Haploid Induction Rates

Doubled haploid technology is a feasible, fast, and cost-efficient way of producing completely homozygous lines in maize. Many factors contribute to the success of this system including the haploid induction rate (HIR) of inducer lines, the inducibility of donor background, and environmental conditions. Sixteen inducer lines were tested on eight different genetic backgrounds of five categories in different environments for the HIR to determine possible interaction specificity. The HIR was assessed using the R1-nj phenotype and corrected using the red root marker or using a gold-standard test that uses plant traits. RWS and Mo-17-derived inducers showed higher average induction rates and the commercial dent hybrid background showed higher inducibility. In contrast, sweet corn and flint backgrounds had a relatively lower inducibility, while non-stiff stalk and stiff stalk backgrounds showed intermediate inducibility. For the poor-performing donors (sweet corn and flint), there was no difference in the HIR among the inducers. Anthocyanin inhibitor genes in such donors were assumed to have increased the misclassification rate in the F-1 fraction and, hence, result in a lower HIR.

Automated summary

The doubled haploid technology is a feasible and cost-efficient way to produce completely homozygous lines in maize, with factors such as haploid induction rate, donor background, and environmental conditions contributing to its success. Different genetic backgrounds showed varied haploid induction rates in different environments, with poor-performing donors possibly affected by anthocyanin inhibitor genes.