Journal
FOODS
Volume 11, Issue 12, Pages -Publisher
MDPI
DOI: 10.3390/foods11121793
Keywords
discarded bread; liquefaction; saccharification reducing sugars; glucose; alpha-amylase; glucoamylase
Categories
Funding
- Junta de Castilla y Leon, Spain [VA177P20]
- FEDER-Interreg Spain-Portugal programme (TRANSCOLAB) [0612_TRANS_CO_LAB_2_P]
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This study investigates the potential use of discarded bread to obtain a high-glucose slurry. Simultaneous hydrolysis using alpha-amylase and glucoamylase was found to reduce time and energy consumption, while achieving similar glucose yield compared to the traditional sequential hydrolysis method.
Bread and bakery products are among the most discarded food products in the world. This work aims to investigate the potential use of wasted bread to obtain a high-glucose slurry. Simultaneous hydrolysis of wasted bread using alpha-amylase and glucoamylase was carried out performing liquefaction and saccharification at the same time. This process was compared with a traditional sequential hydrolysis. Temperature and pH conditions were optimized using a response surface design determining viscosity, reducing sugars and glucose concentration during the enzymatic processes. The optimal conditions of pH and temperature in the saccharification stage and the simultaneous hydrolysis were pretty similar. Results show that the slurry produced with simultaneous process had a similar glucose yield at 2 h, and at 4 h a yield higher than that obtained by the sequential method of 4 h and could reduce time and energy.
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