Journal
PATHOGENS
Volume 11, Issue 8, Pages -Publisher
MDPI
DOI: 10.3390/pathogens11080861
Keywords
tuberose; TuMMV; TuMMoV; high-throughput sequencing; phylogenetic analysis
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Funding
- Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, Uttar Pradesh
- Council of Science & Technology, Uttar Pradesh
- Bioinformatics facility, Department of Biotechnology, India
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This study reports the coding-complete genomic RNA of tuberose mild mosaic virus (TuMMV) and tuberose mild mottle virus (TuMMoV) and confirms their presence using RT-PCR assays. These viruses are widely distributed in tuberose growing countries with low biological incidence. Further studies are needed to understand the potential threat of these viruses to tuberose production.
Tuberose (Polianthes tuberosa) is an ornamental flowering crop of the Amaryllidaceae family. Tuberose mild mosaic virus (TuMMV) and tuberose mild mottle virus (TuMMoV), members of the genus Potyvirus, are ubiquitously distributed in most tuberose growing countries worldwide with low biological incidence. Here, we report the first coding-complete genomic RNA of TuMMV and TuMMoV obtained through high-throughput sequencing (HTS) and further, the presence of both the viruses were confirmed using virus-specific primers in RT-PCR assays. Excluding the poly (A) tail, the coding-complete genomic RNA of TuMMV and TuMMoV was 9485 and 9462 nucleotides (nts) in length, respectively, and contained a single large open reading frame (ORF). Polyprotein encoded by both the viral genomes contained nine putative cleavage sites. BLASTn analysis of TuMMV and TuMMoV genomes showed 72.40-76.80% and 67.95-77% nucleotide sequence similarities, respectively, with the existing potyviral sequences. Phylogenetic analysis based on genome sequences showed that TuMMV and TuMMoV clustered in a distinct Glade to other potyviruses. Further studies are required to understand the mechanism of symptom development, distribution, genetic variability, and their possible threat to tuberose production in India.
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