Quantitative Cryo-Electron Tomography

The three-dimensional organization of biomolecules important for the functioning of all living systems can be determined by cryo-electron tomography imaging under native biological contexts. Cryo-electron tomography is continually expanding and evolving, and the development of new methods that use the latest technology for sample thinning is enabling the visualization of ever larger and more complex biological systems, allowing imaging across scales. Quantitative cryo-electron tomography possesses the capability of visualizing the impact of molecular and environmental perturbations in subcellular structure and function to understand fundamental biological processes. This review provides an overview of current hardware and software developments that allow quantitative cryo-electron tomography studies and their limitations and how overcoming them may allow us to unleash the full power of cryo-electron tomography.

Automated summary

This article reviews the hardware and software developments in quantitative cryo-electron tomography, discusses its importance and limitations in biological research. Quantitative cryo-electron tomography can be used to visualize the three-dimensional structure of biological systems, helping us understand changes in cellular structure and function.