Performance of Dried Blood Spot Samples in SARS-CoV-2 Serolomics

Article Multidisciplinary Sciences

Using sero-epidemiology to monitor disparities in vaccination and infection with SARS-CoV-2

Isobel Routledge et al.

Summary: This study analyzes data from a serological surveillance platform in San Francisco and finds significant variation in infection and vaccination history by race/ethnicity and socioeconomic status.

NATURE COMMUNICATIONS (2022)

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Review Infectious Diseases

SARS-CoV-2 seroprevalence worldwide: a systematic review and meta-analysis

Ali Rostami et al.

Summary: The study revealed significant variations in SARS-CoV-2 seroprevalence among different geographic regions, possibly related to income levels, human development indices, geographic latitudes, and climates. It was estimated that approximately 263.5 million individuals had been exposed or infected globally by 2020.

CLINICAL MICROBIOLOGY AND INFECTION (2021)

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Article Genetics & Heredity

COVID-ONE-hi: The One-stop Database for COVID-19-specific Humoral Immunity and Clinical Parameters

Zhaowei Xu et al.

Summary: The database COVID-ONE-hi provides an analysis of COVID-19-specific IgG/IgM immune responses and clinical parameters, based on data from 783 COVID-19 patients. It offers a comprehensive analysis report for further interpretation and is freely available for use at www.COVID-ONE.cn.

GENOMICS PROTEOMICS & BIOINFORMATICS (2021)

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Review Multidisciplinary Sciences

Reliability of dried blood spot (DBS) cards in antibody measurement: A systematic review

Fahimah Amini et al.

Summary: This systematic review found a good correlation between antibody concentrations in dried blood spots (DBS) and serum/plasma samples, supporting their wider use in vaccine and sero-epidemiological studies. There is limited data on anti-bacterial antibodies and proper storage of DBS is crucial for longer term storage.

PLOS ONE (2021)

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Article Virology

From Multiplex Serology to Serolomics-A Novel Approach to the Antibody Response against the SARS-CoV-2 Proteome

Julia Butt et al.

Summary: The emergence of the SARS-CoV-2 pandemic has highlighted the urgent need for specific and sensitive high-throughput serological assays to assess the epidemiology of SARS-CoV-2. Researchers have developed a fluorescent-bead based SARS-CoV-2 multiplex serology assay to detect antibody responses to the SARS-CoV-2 proteome. This assay demonstrated high sensitivity and specificity in COVID-19 patients, allowing for epidemiological research and explorative analyses regarding disease progression and antibody response.

VIRUSES-BASEL (2021)

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Article Immunology

SARS-CoV-2 Proteome-Wide Analysis Revealed Significant Epitope Signatures in COVID-19 Patients

Tatjana Schwarz et al.

Summary: The WHO declared the COVID-19 outbreak as a public health emergency of international concern, with the newly emerged coronavirus, SARS-CoV-2, causing a range of disease outcomes from asymptomatic infection to severe pneumonia. Understanding the host immune response is crucial for intervention strategies, with humoral responses playing a significant role in defending viral infections.

FRONTIERS IN IMMUNOLOGY (2021)

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Article Multidisciplinary Sciences

Spread of a SARS-CoV-2 variant through Europe in the summer of 2020

Emma B. Hodcroft et al.

Summary: A variant of SARS-CoV-2, 20E (EU1), was identified in Spain in early summer 2020 and later spread across Europe. Despite not showing increased transmissibility, the variant's success can be attributed to rising incidence in Spain, resumption of travel, and lack of effective screening and containment measures. Travel played a significant role in introducing the variant multiple times to European countries during the summer, undermining local efforts to prevent SARS-CoV-2 infection.

NATURE (2021)

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Article Multidisciplinary Sciences

Multianalyte serology in home-sampled blood enables an unbiased assessment of the immune response against SARS-CoV-2

Niclas Roxhed et al.

Summary: Researchers have developed a multiplexed approach to screen IgG and IgM levels against several SARS-CoV-2 proteins in home-sampled dried blood spots and estimated a seroprevalence of 12.5% in Stockholm in spring of 2020.

NATURE COMMUNICATIONS (2021)

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Article Biochemistry & Molecular Biology

Dynamic landscape mapping of humoral immunity to SARS-CoV-2 identifies non-structural protein antibodies associated with the survival of critical COVID-19 patients

Linlin Cheng et al.

Summary: This study conducted a longitudinal analysis of antibody responses to SARS-CoV-2 proteins in critical COVID-19 patients, revealing that binding epitopes of IgM and IgG antibodies differ and are mostly located within nonstructural proteins. IgM antibodies targeting nsp3 and nsp5 proteases are associated with a good prognosis, whereas IgG antibodies targeting structural proteins are associated with high mortality. Validation of antibody epitopes in patients with high mortality rate was performed using an independent serum cohort, providing insights for antibody-based COVID-19 treatment.

SIGNAL TRANSDUCTION AND TARGETED THERAPY (2021)

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Letter Immunology