Journal
ADVANCED FUNCTIONAL MATERIALS
Volume 25, Issue 17, Pages 2573-2582Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adfm.201500299
Keywords
-
Categories
Funding
- National Research Foundation of Korea - Korean Government [2012M3A9C6049835, 2014M3A9B6034223]
Ask authors/readers for more resources
2D/3D hybrid cell culture systems are constructed by increasing the temperature of the thermogelling poly(ethylene glycol)-poly(L-alanine) diblock copolymer (PEG-L-PA) aqueous solution in which tonsil tissue-derived mesenchymal stem cells and graphene oxide (GO) or reduced graphene oxide (rGO) are suspended, to 37 degrees C. The cells exhibit spherical cell morphologies in 2D/3D hybrid culture systems of GO/PEG-L-PA and rGO/PEG-L-PA by using the growth medium. The cell proliferations are 30%-50% higher in the rGO/PEG-L-PA hybrid system than in the GO/PEG-L-PA hybrid system. When chondrogenic culture media enriched with TGF-beta 3 is used in the 2D/3D hybrid systems, cells extensively aggregate, and the expression of chondrogenic biomarkers of SOX 9, COL II A1, COL II, and COL X significantly increases in the GO/PEG-L-PA 2D/3D hybrid system as compared with the PEG-L-PA 3D systems and rGO/PEG-L-PA 2D/3D hybrid system, suggesting that the GO/PEG-L-PA 2D/3D hybrid system can be an excellent candidate as a chondrogenic differentiation platform of the stem cell. This paper also suggests that a 2D/3D hybrid system prepared by incorporating 2D materials with various surface biofunctionalities in the in situ forming 3D hydrogel matrix can be a new cell culture system.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available