Journal
BIOMOLECULES
Volume 12, Issue 7, Pages -Publisher
MDPI
DOI: 10.3390/biom12071002
Keywords
platelet-rich plasma; PRP; plasma rich in growth factors; PRGF; topical serum; proteomics; regenerative medicine; skin
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Funding
- Basque Country Government, within the Elkartek program [KK-2019/00094]
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The composition differences between autologous topical serum (ES) and leukocyte- and platelet-rich plasma (L-PRP) lead to different responses in human dermal fibroblasts (HDFs). ES treatment promotes protein synthesis, cell proliferation, and migration, while L-PRP treatment induces a response similar to proinflammatory molecules.
Platelet-rich plasma (PRP) is nowadays used in the treatment of different types of cutaneous lesions. However, different compositions can influence clinical outcomes. Among them, the inclusion of leukocytes is controversial. High-throughput proteomics techniques were used to analyze the proteins that are differentially expressed in human dermal fibroblasts (HDFs) after treatment for 24 h with two PRP types, autologous topical serum (Endoret serum-ES) derived from plasma rich in growth factors (PRGF) and leukocyte- and platelet-rich plasma (L-PRP). The identified proteins were then classified by both Gene Ontology and Ingenuity Pathway Analysis. The obtained results show that the compositions of ES and L-PRP differ in such a way that they induce different responses in HDFs. ES-treated HDFs overexpress growth factor-related proteins, leading to protein synthesis, cell proliferation and migration. By contrast, L-PRP treatment induces a response similar to that caused by proinflammatory molecules. These data could explain the contradictory clinical results obtained for the different types of PRP, especially with respect to their leukocyte contents.
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