Self-Assembling Protein Surfaces for In Situ Capture of Cell-Free-Synthesized Proteins

We present a new method for the surface capture of proteins in cell-free protein synthesis (CFPS). We demonstrate the spontaneous self-assembly of the protein BslA into functionalizable surfaces on the surface of a CFPS reaction chamber. We show that proteins can be covalently captured by such surfaces, using Catcher/Tag technology. Importantly, proteins of interest can be captured either when synthesised in situ by CFPS above the BslA surfaces, or when added as pure protein. The simplicity and cost efficiency of this method suggest that it will find many applications in cell-free-based methods.

Automated summary

This article presents a new method for surface capture of proteins in cell-free protein synthesis (CFPS), demonstrating the spontaneous self-assembly of protein BslA into functionalizable surfaces. The method allows for the covalent capture of proteins using Catcher/Tag technology. The simplicity and cost efficiency of this method suggest its potential applications in cell-free-based techniques.