4.3 Article

The complete chloroplast genome of Coffea liberica (Gentianales: Rubiaceae)

Journal

MITOCHONDRIAL DNA PART B-RESOURCES
Volume 7, Issue 8, Pages 1454-1456

Publisher

TAYLOR & FRANCIS LTD
DOI: 10.1080/23802359.2022.2107459

Keywords

Coffea liberica; chloroplast genome; phylogenetic tree

Funding

  1. Program of Hainan Association for Science and Technology Plans to Youth RD Innovation [QCXM202005]
  2. Major Science and Technology Projects of Yunnan Province [2018ZG016]
  3. Program of Yunnan Province Technology Hall Plans to cultivation Innovation guidance and technology enterprise [202204BI090009]
  4. innovation platform for Academicians of Hainan Province

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This study investigated the chloroplast genome of Liberian coffee and found it to be closely related to C. canephora.
Coffee is one of the most popular beverages around the world. As one of the best-known coffee species, Liberian coffee (Coffea liberica Bull ex Hiern 1876) has a high resistance to leaf rust, a devasting disease caused by Hemileia vastatrix. However, there are few reports on the systematic position and phylogenetic relationship of C. liberica at the chloroplast (cp) genome level. Thus, we successfully assembled its cp genome. The full length is 154,799 bp with a GC content of 37.48%. We have further annotated the cp genome and predicted 85 protein-coding genes together with 8 rRNAs and 37 tRNAs. Furthermore, a large single copy region (LSC), a small single copy region (SSC), an inverted repeat region a (IRa) and an inverted repeat region b (IRb) are identified with lengths of 84,868 bp, 18,121 bp, 25,905 bp and 25,905 bp, respectively. The phylogenetic tree indicates that C. liberica is closely related to C. canephora, which is consistent with a previous result obtained from genotyping-by-sequencing.

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